Protein Info for PFR28_00194 in Pseudomonas sp. RS175

Annotation: Bifunctional enzyme CysN/CysC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 23 to 228 (206 residues), 162.4 bits, E=2e-51
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 25 to 432 (408 residues), 687.5 bits, E=9.9e-211
• PF01926: MMR_HSR1
  • amino acids 31 to 166 (136 residues), 25.2 bits, E=3e-09
• TIGR00231: small GTP-binding protein domain
  • amino acids 31 to 211 (181 residues), 51.6 bits, E=1.4e-17
• TIGR00455: adenylyl-sulfate kinase
  • amino acids 450 to 626 (177 residues), 140 bits, E=1e-44
• PF01583: APS_kinase
  • amino acids 463 to 612 (150 residues), 170.3 bits, E=6.2e-54

Best Hits

Swiss-Prot: 87% identical to CYSNC_PSEAE: Bifunctional enzyme CysN/CysC (cysNC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00955, bifunctional enzyme CysN/CysC [EC: 2.7.1.25 2.7.7.4] (inferred from 98% identity to pba:PSEBR_a4725)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (4/12 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.25, 2.7.7.4

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Protein Sequence (632 amino acids)

>PFR28_00194 Bifunctional enzyme CysN/CysC (Pseudomonas sp. RS175)
MSHQSDLISEDILAYLGQHERKELLRFLTCGNVDDGKSTLIGRLLHDSKMIYEDHLEAIT
RDSKKVGTTGDDIDLALLVDGLQAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
MATGASTCDLAIILVDARYGVQTQTRRHSFIASLLGIKHIVVAINKMDLKDFDQGVFESI
KADYLKFAEGLKMKPTTLHFVPMSALKGDNVVNKSQRSPWYTGQSLMEILETVEVAGDRN
FTDLRFPVQYVNRPNLNFRGFAGTLASGIVHKGDEVVVLPSGKSSRVKSIVTFEGELEHA
GPGQAVTLTMEDEIDISRGDLLVHADNVPPVTDSFEAMLVWMAEEPMLPGKKYDIKRATS
YVPGSIASIVNRVDVNTLEEGPASALQLNEIGKVKIALDAPIALDGYESNRTTGAFIVID
RLTNGTVGAGMIVAQPLTHGSSTHHGKLAHVSVEERTQRFGQQPATVLFSGLSGAGKSTL
AYAVERKLFDMGRAVFVLDGQNLRHDLNKGLPQDRAGRTENWRRAAHVARQFNEAGLLTL
AAFVAPSAEGREQAKDLIGRDRLLTVYVQASPTVCAQRDPQGLYAAAGDNIPGESFPYDV
PLDADLVIDTQALTLEEGVKQVLDLLRKRGAI