Protein Info for PFR28_00013 in Pseudomonas sp. RS175

Annotation: Methylmalonate-semialdehyde dehydrogenase [acylating]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01722: methylmalonate-semialdehyde dehydrogenase (acylating)
  • amino acids 15 to 491 (477 residues), 759.5 bits, E=6.9e-233
• PF00171: Aldedh
  • amino acids 23 to 487 (465 residues), 494.4 bits, E=1.4e-152

Best Hits

Swiss-Prot: 61% identical to MMSA_AEDAE: Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial (AAEL001134) from Aedes aegypti

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 97% identity to pba:PSEBR_a689)

MetaCyc: 72% identical to methylmalonate-semialdehyde dehydrogenase subunit (Burkholderia cepacia R34)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)

MetaCyc Pathways

• myo-inositol degradation I (6/7 steps found)
• β-alanine degradation II (2/2 steps found)
• β-alanine degradation I (1/2 steps found)
• propanoyl-CoA degradation II (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• myo-, chiro- and scyllo-inositol degradation (6/10 steps found)
• 2,4-dinitrotoluene degradation (1/7 steps found)

KEGG Metabolic Maps

• Inositol phosphate metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.27

Use Curated BLAST to search for 1.2.1.27

Protein Sequence (508 amino acids)

>PFR28_00013 Methylmalonate-semialdehyde dehydrogenase [acylating] (Pseudomonas sp. RS175)
MNVSLTPSDTTLQTVKLLIDGEWVESRSNEWHDIINPATQQVLAKVPFATATEVESAIAA
AQRAFQTWKLTPIGARMRIMLKLQALIREHSKRIAAVLSAEQGKTLADAEGDIFRGLEVV
EHACSIGTLQMGEFAENVAGGVDTYTLRQPIGVCAGITPFNFPAMIPLWMFPMAIACGNT
FVLKPSEQDPLSTMLLVELAIEAGVPAGVLNVVHGGKDVVDALCTHKDIKAVSFVGSTAV
GTHVYELAGRHGKRVQSMMGAKNHAVVLPDANREQTLNALVGAGFGAAGQRCMATSVVVM
VGAARQWLPELKALAQKLKVNAGSEPGTDIGPVISKRAKARILELIESGVNEGAKLELDG
RDISVPGFEQGNFVGPTLFSGVTTDMRIYTEEIFGPVLVVLEVDTLDQAIALVNANPFGN
GTGLFTQSGAAARKFQSEIDVGQVGINIPIPVPVPFFSFTGSRGSKLGDLGPYGKQVVQF
YTQTKTVTARWFDDDSINDGVNTTINLR