Protein Info for PFR28_00010 in Pseudomonas sp. RS175

Annotation: Diguanylate cyclase DgcN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 transmembrane" amino acids 21 to 46 (26 residues), see Phobius details amino acids 151 to 175 (25 residues), see Phobius details PF17152: CHASE8" amino acids 43 to 143 (101 residues), 107.4 bits, see alignment E=4e-35 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 243 to 404 (162 residues), 148.4 bits, see alignment E=7.8e-48 PF00990: GGDEF" amino acids 247 to 401 (155 residues), 156.5 bits, see alignment E=5e-50

Best Hits

KEGG orthology group: None (inferred from 92% identity to pba:PSEBR_a686)

Predicted SEED Role

"Inner membrane protein YfiN"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (416 amino acids)

>PFR28_00010 Diguanylate cyclase DgcN (Pseudomonas sp. RS175)
MSLSTPRILPTLGSVIGRGHLIVALVAIAMASISLTLLGVLALRVYADHNLHLIARSINY
TVEAAVVFDDSAAATEALALIASTEEVADAQVFNEHGRLLARWQRPETGLLSDLEMHIAK
AFLEKPISLPIVHQGQAIGSVLLAGHGGSLLRFLLSGLAGIILCTAVSAWMALYLARRQL
RAITGPLRCLAEVAHAARSERALDRRVPPAAIAELDNLGNDFNALLAELESWQTHLQSEN
ESLAHQASHDSLTGLPNRAYFEGRLIRALRNASKLDEQVAVLYLDSDRFKGINDNFGHAA
GDAVLTAVATRVRAQLREDDLVARLGGDEFAVLLSPLHKLEDAEMIAEKIIASMEMPIQL
PGNASVLTSLSIGIAVYPEHGTTPGALLHAADAAMYQAKRLARGGRHSSGAEHPVA