Protein Info for PFR28_00007 in Pseudomonas sp. RS175

Annotation: RecBCD enzyme subunit RecB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1229 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR00609: exodeoxyribonuclease V, beta subunit" amino acids 9 to 1211 (1203 residues), 1095.6 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 11 to 160 (150 residues), 78.4 bits, see alignment E=1.9e-25 amino acids 335 to 443 (109 residues), 90.3 bits, see alignment E=4.4e-29 PF13245: AAA_19" amino acids 18 to 433 (416 residues), 41.4 bits, see alignment E=4.1e-14 PF13361: UvrD_C" amino acids 704 to 841 (138 residues), 57.1 bits, see alignment E=5.4e-19 PF12705: PDDEXK_1" amino acids 1066 to 1211 (146 residues), 27.5 bits, see alignment E=7.4e-10

Best Hits

KEGG orthology group: K03582, exodeoxyribonuclease V beta subunit [EC: 3.1.11.5] (inferred from 88% identity to pba:PSEBR_a683)

Predicted SEED Role

"Exodeoxyribonuclease V beta chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1229 amino acids)

>PFR28_00007 RecBCD enzyme subunit RecB (Pseudomonas sp. RS175)
MSRQTPLALAFPLHGSQLIEASAGTGKTFTISALYLRLVLGHGGPATGFGRELLPPQILV
VTFTDAATKELRERIRTRLAEAARFFRDEIPAPDSLIVQLRGEFAEEHWPACANRLDIAA
QWMDEAAVSTIHSWCQRMLREHAFDSGSLFTQTLETDHSELLGEVLRDYWRMFCYSMQGE
ALNWVRAHWGGPASLLPRVKGLFASEHGNGEGPEPAELIAASLRERSAALLDLKAPWRQW
AVELLEICQQGVANKLVDARKMQARYFEPWFQKISAWVEDESVELLDIGTGFTRLTPEGM
AQAWKGEVPSHPGLDAMVGLKASLEALPTPDAAVLQHAAQWVGARFEAEKRRRAEMGFDD
MLLRLDAALQAEGGERLASLIREQFPVALIDEFQDTDPVQYRIFESIYRIEGNNPETGLF
LIGDPKQAIYAFRGADIHTYLRARVATAGRLHTLGTNFRSSHAMVGAVNHVFQYAESRPQ
GRGAFLFREASGNNPVPFLPVESQGRNEFLQIAGQAVTAMNIWHLPSEQPLSGAVYRQNM
AAACASAITGLLNGGQAGRNGFMDPESVLRRLLPADIAILVRDGKEAQAVRDELSARGVR
SVYLSDKDSVFASQEARDLLIWLKACAEPDVERPLRAALASITLNLPLTELERLNQDELV
WEARVMQFRGYRTVWRTQGVLPMLRRLLHDFELPQALMARSDGERVLTNLLHLCELLQQA
AGELDGEQALIRHLSEHLALSGQAAEEQILRLESDEQLVKVVTIHKSKGLEYPLVFLPFI
CSTKPVDGTRLPLHYHDESGRAQVSLRPTVELIARADEERLAEDLRLLYVALTRAQHACW
LGVADLKRGSNNTSILHLSALGYLLGGGTPLADSVELKRWLEDLQQGSATVSCQEMPLAT
DEHYQPPRSEAVLREPLKPARKASENWWIASYSALRIGESLSAGSDAAPEDPQAQKLFDD
ERLDPEAPRAVAVAGGDIHRFPRGPNPGTFLHGLLEWIAGEGFAAAPSVIEDVIARRCNR
RGWKGWITTLNDWLQHLVALPLQIGSNQAPVVLAHLDQFQVEMEFWFASHKVDVLKLDQL
VCQFTHGGVARVAAEPVLLNGMFKGFIDLTFEHDGRYYVADYKSNWLGADDAAYTSQAME
QSILDNRYDLQYVLYLLALHRQLKARLPDYDYDRHVGGALYLFLRGTRATSQGVYFTRPA
RALIEQLDRLFQGVPEPKAEPAWVQGELL