Protein Info for PFLU_RS30110 in Pseudomonas fluorescens SBW25

Annotation: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 4 to 451 (448 residues), 641.2 bits, E=4.7e-197
• PF12804: NTP_transf_3
  • amino acids 6 to 120 (115 residues), 73.3 bits, E=5.5e-24
• PF00483: NTP_transferase
  • amino acids 6 to 204 (199 residues), 44.9 bits, E=2.2e-15
• PF00132: Hexapep
  • amino acids 263 to 296 (34 residues), 31.3 bits, 2.4e-11
  • amino acids 392 to 425 (34 residues), 33.8 bits, 3.7e-12
• PF14602: Hexapep_2
  • amino acids 281 to 312 (32 residues), 18.5 bits, see alignment (E = 2.6e-07)
  • amino acids 392 to 425 (34 residues), 28.9 bits, 1.6e-10

Best Hits

Swiss-Prot: 100% identical to GLMU_PSEFS: Bifunctional protein GlmU (glmU) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 100% identity to pfs:PFLU6116)

MetaCyc: 54% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• peptidoglycan recycling I (11/14 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (9/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See C3K1E4 at UniProt or InterPro

Protein Sequence (455 amino acids)

>PFLU_RS30110 UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (Pseudomonas fluorescens SBW25)
MSLEIVILAAGQGTRMRSALPKVLHPVAGNSMLGHVIHSARQLDPQRIHVVIGHGADVVR
ERLAADDLNFVLQDKQLGTGHATAQAVPFITADTVLILYGDVPLIEVETLQRLLKHVVPG
QMGLLTVELDDPTGYGRIVRNADGKVAAIVEHKDASEAQRAITEGNTGILAVPANKLADW
MSRLSNNNAQGEYYLTDVIEMAVSDGLLVATEQPHDPMEVQGANDRKQLAELERHYQLRE
GRRLMAQGVTLRDPARFDVRGEVSVGRDVLIDINVILEGRVIIEDDVVIGPNCVIKDSTL
RKGAVIKANSHIDGAVMGEGSDAGPFARLRPGTVMGARAHVGNFVELKNAKMGDDAKAGH
LAYLGDAVIGARSNIGAGAITCNYDGANKYQTTIGEDVFIGSNNSLIAPVTIGDGSNTAA
GSTINQDVDKSQLAVARARQRNIDGWKRPVKIKKT