Protein Info for PFLU_RS30060 in Pseudomonas fluorescens SBW25

Annotation: bifunctional alpha/beta hydrolase/class I SAM-dependent methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 584 PF12146: Hydrolase_4" amino acids 31 to 267 (237 residues), 244.4 bits, see alignment E=2e-76 PF00561: Abhydrolase_1" amino acids 36 to 154 (119 residues), 42.7 bits, see alignment E=1.1e-14 PF12697: Abhydrolase_6" amino acids 49 to 257 (209 residues), 34.5 bits, see alignment E=7.2e-12 PF12147: Methyltransf_20" amino acids 276 to 583 (308 residues), 509.9 bits, see alignment E=4.8e-157

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU6106)

Predicted SEED Role

"Putative lipase in cluster with Phosphatidate cytidylyltransferase" in subsystem Triacylglycerol metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K1D4 at UniProt or InterPro

Protein Sequence (584 amino acids)

>PFLU_RS30060 bifunctional alpha/beta hydrolase/class I SAM-dependent methyltransferase (Pseudomonas fluorescens SBW25)
MREQQEHTFSTHDGVELFYRHWPAAAPADGEPRKAIVLFHRGHEHSGRIAHLVDELNLPQ
FDFFAWDARGHGQSPGERGDSPSFATSARDVQTFCDHIGTTYGIEEEHLAVIAQSVGAVI
AATWVHDYAPKIRALVLASPAFKVKLYVPFARPGLALMRKFRGNFFVNSYVKAKFLSHDP
ERVASYDSDPLITKAISVNVLLGLYEAADRVVADAQAIQVPTQLLVSGSDFVVHRKPQQQ
FFDRLGSLKKELHILPGFFHDTLGERDRATAVNSARRFILQNFEHPLDRASLLDADKLGA
TCVESEALAAPLPRNSLRDLYWRLTRASMGLGKNLSDGVKLGFDTGFDSGSTLDYVYRNT
PTGKGGLGRMIDTNYLNSIGWRGIRQRKLNVEELLRLAMATLRDEQREVRIVDIAAGHGR
YILEALQGVSPLPESILLRDYSDINVRDGGALIREKGLGDIAQFVKGDAFDRLDLAALDP
KPTLAVVSGLYELFADNAMVGGSLAGLAEAVEPGGYLVYTGQPWHPQLELIARALTSHRQ
GQAWVMRRRSQAEMDQLVEAAGFRKITQRVDEWGIFTVSLAQKI