Protein Info for PFLU_RS29930 in Pseudomonas fluorescens SBW25

Annotation: pyridoxal kinase PdxY

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00687: pyridoxal kinase
  • amino acids 6 to 288 (283 residues), 359.7 bits, E=6e-112
• PF08543: Phos_pyr_kin
  • amino acids 71 to 256 (186 residues), 39.5 bits, E=4.3e-14
• PF00294: PfkB
  • amino acids 135 to 263 (129 residues), 27.5 bits, E=2e-10

Best Hits

Swiss-Prot: 100% identical to PDXY_PSEFS: Pyridoxal kinase PdxY (pdxY) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00868, pyridoxine kinase [EC: 2.7.1.35] (inferred from 100% identity to pfs:PFLU6081)

MetaCyc: 55% identical to pyridoxal kinase 2 (Escherichia coli K-12 substr. MG1655)
Pyridoxal kinase. [EC: 2.7.1.35]

Predicted SEED Role

"Pyridoxal kinase (EC 2.7.1.35)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 2.7.1.35)

MetaCyc Pathways

• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (11/12 steps found)
• pyridoxal 5'-phosphate salvage I (5/6 steps found)
• pyridoxal 5'-phosphate salvage II (plants) (5/9 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (4/8 steps found)

KEGG Metabolic Maps

• Vitamin B6 metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.35

Sequence Analysis Tools

See C3K4G7 at UniProt or InterPro

Protein Sequence (290 amino acids)

>PFLU_RS29930 pyridoxal kinase PdxY (Pseudomonas fluorescens SBW25)
MKRTPHLLAIQSHVVFGHAGNSAAVFPMQRVGVNVWPLNTVQFSNHTQYGQWAGEVLAPQ
QIPALVEGIAAIGELGNCDAILSGYLGSADQGRAILTGVARIKAINPKALYLCDPVMGHP
EKGCIVPQEVSDFLLDEAAAMADFLCPNQLELDSFAGRKPQSLFDCLAMAKALLARGPKA
VLVKHLDYPGKLPDGFEILLVTAEGSWHLRRPLLAFARQPVGVGDLTSGLFLARVLLGDS
LLAAFEFTAAAVHEVLLETQACASYELELVRAQDRIAHPRVRFEATPIEL