Protein Info for PFLU_RS29895 in Pseudomonas fluorescens SBW25

Annotation: EAL domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 958 transmembrane" amino acids 34 to 57 (24 residues), see Phobius details amino acids 66 to 89 (24 residues), see Phobius details amino acids 102 to 124 (23 residues), see Phobius details amino acids 130 to 149 (20 residues), see Phobius details amino acids 155 to 174 (20 residues), see Phobius details amino acids 180 to 200 (21 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 304 to 401 (98 residues), 30.7 bits, see alignment E=2.9e-11 amino acids 400 to 523 (124 residues), 56.7 bits, see alignment E=2.7e-19 PF08447: PAS_3" amino acids 305 to 388 (84 residues), 69.4 bits, see alignment E=9.3e-23 amino acids 426 to 510 (85 residues), 28.3 bits, see alignment E=6.3e-10 PF00989: PAS" amino acids 407 to 513 (107 residues), 38 bits, see alignment E=5.4e-13 PF08448: PAS_4" amino acids 409 to 519 (111 residues), 48.5 bits, see alignment E=3.2e-16 PF13426: PAS_9" amino acids 414 to 515 (102 residues), 46.3 bits, see alignment E=1.6e-15 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 527 to 686 (160 residues), 106.5 bits, see alignment E=1.2e-34 PF00990: GGDEF" amino acids 529 to 684 (156 residues), 141 bits, see alignment E=1.1e-44 PF00563: EAL" amino acids 705 to 940 (236 residues), 257 bits, see alignment E=5.4e-80

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU6074)

Predicted SEED Role

"Sensory box/GGDEF family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4G0 at UniProt or InterPro

Protein Sequence (958 amino acids)

>PFLU_RS29895 EAL domain-containing protein (Pseudomonas fluorescens SBW25)
MTLSTDLFGPSAAPAQVLRKHYATEMAVERTRLLYQGSLLPTLLMLVNGLVCAWLLWNPK
QYLLDSIWLVWLLALVAMRVIQVAAFDSAMPSRQAQPVWRRMFMLGSAVSGLTLATAAIA
LVPADSFMQQAWVFGLIGAATLSASVAYAVSVPAFLSFALPCLVPSIIYLFWSGDPQQRG
WGVLGLILLASLSLVAWQVNRLIQRGLLRRFQNQALIEHLQQAQQRSEQLNQELVREVEQ
RRQVEQELREAQVGLQDRVAQRSQELDAASLALNKSEARLAMALQASELGLWDWNLQTDE
VHHTQLKELFGLEPEYVTAMLGHLKPRLHPEDLPLLKRALVEHLKGRSEDYQVEYRVRHG
DGHWVWIEDRGRAVERAPSGRVTRMLGTRRDISASKALEEQQRLASTVFEAASEGIVILD
PDYVLIAVNQAFSRVTGFETDDMIGRNVVELPSSRDARRHFPVIRQALLSHGTWQGELVE
TRKNGELYPQWLQLNVVRDVRGKVSHIVGFFADLSARRESEERMRYLTHYDELTGLANRS
LFRERLREAHQRVRQGGRSLALLHINLDRFKLLNDSLGHEVADQLLQKMARRLINALPEA
DTIARLSGDEFAVLFDAYGNLSSLARVATRLLAKLRVPVTVEGHELVVSASMGVSLLPDN
AREISALVSQSNMAMQHAKHLGGNNFQFYTDSLQASTLERLQLENHLRKAIEERQLSVFY
QPKLCLATGKLNAAEALIRWEHPQWGMVPPGDFIGLAEETGLIVPLGEFVLREACWQACE
WQRQGLAPIRVSVNLSVHQLRQGKLVSLVRQVLEETGLDPQYLELELTESQLLDSVEHII
ATFQQLRDLGVKLAIDDFGTGYSSLSYLKRIPVDYVKIDQTFIRGLGQGREDAAITRAII
AMAHGLALKVVAEGVEDQQQLDFLRGERCDEVQGYLISRPMQAEGLADLLRKNADFPE