Protein Info for PFLU_RS29710 in Pseudomonas fluorescens SBW25

Annotation: chorismate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 79% identical to UBIC2_PSEPF: Probable chorismate pyruvate-lyase 2 (ubiC2) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03181, chorismate--pyruvate lyase [EC: 4.1.3.40] (inferred from 100% identity to pfs:PFLU6035)

Predicted SEED Role

"Chorismate--pyruvate lyase (EC 4.1.3.40)" in subsystem Ubiquinone Biosynthesis (EC 4.1.3.40)

MetaCyc Pathways

superpathway of ubiquinol-8 biosynthesis (early decarboxylation) (10/12 steps found)
4-hydroxybenzoate biosynthesis II (bacteria) (1/1 steps found)
superpathway of chorismate metabolism (42/59 steps found)
polybrominated phenols biosynthesis (1/4 steps found)
ubiquinol-8 biosynthesis (late decarboxylation) (4/9 steps found)
spongiadioxin C biosynthesis (2/7 steps found)
polybrominated dihydroxylated diphenyl ethers biosynthesis (2/8 steps found)
p-HBAD biosynthesis (1/9 steps found)
tetrahydromethanopterin biosynthesis (3/14 steps found)
superpathway of polybrominated aromatic compound biosynthesis (2/20 steps found)
phenolphthiocerol biosynthesis (1/23 steps found)

KEGG Metabolic Maps

Ubiquinone and menaquinone biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4C3 at UniProt or InterPro

Protein Sequence (187 amino acids)

>PFLU_RS29710 chorismate lyase (Pseudomonas fluorescens SBW25)
MPHSIVPPDACQWLIQSLLTPAPAPLTLNWLFNEDSLTRRLTWLSNDGFSVTPLFEGWQP
LRDDECAALALAPSSIGWVREVYLRGNGQPWVFARSVAARSALQGDGLHMDELGSRSLGE
LLFCDQAFTRQAIEVCHYPRHWLPTADQVDGLWGRRSRFDRGPLSVLVAEIFLPSFWHAL
HAHPENC