Protein Info for PFLU_RS29630 in Pseudomonas fluorescens SBW25

Annotation: type VI secretion system contractile sheath small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 168 TIGR03358: type VI secretion protein, VC_A0107 family" amino acids 7 to 162 (156 residues), 186 bits, see alignment E=2.1e-59 PF05591: T6SS_VipA" amino acids 11 to 161 (151 residues), 192.6 bits, see alignment E=1.9e-61

Best Hits

KEGG orthology group: K11901, type VI secretion system protein ImpB (inferred from 100% identity to pfs:PFLU6019)

Predicted SEED Role

"Uncharacterized protein ImpB"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4A7 at UniProt or InterPro

Protein Sequence (168 amino acids)

>PFLU_RS29630 type VI secretion system contractile sheath small subunit (Pseudomonas fluorescens SBW25)
MAKQSSQKFIARNRAPRVQIEYDVELYGAEKKVQLPFVMGVMADLAGKPAEPLAPVADRK
FLEVDVDNFDSRLKAMQPRVAFHVPNELTGEGNLSLDITFESMDDFSPAAVARKVDSLNQ
LLEARTQLANLLTYMDGKTGAEEIIMKAIKDPALLQALASAPKPAGDQ