Protein Info for PFLU_RS29530 in Pseudomonas fluorescens SBW25

Annotation: tonB-system energizer ExbB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 322 signal peptide" amino acids 1 to 40 (40 residues), see Phobius details transmembrane" amino acids 105 to 127 (23 residues), see Phobius details amino acids 213 to 235 (23 residues), see Phobius details amino acids 256 to 279 (24 residues), see Phobius details TIGR02797: tonB-system energizer ExbB" amino acids 94 to 303 (210 residues), 347.4 bits, see alignment E=1.6e-108 PF01618: MotA_ExbB" amino acids 170 to 285 (116 residues), 109.9 bits, see alignment E=3.8e-36

Best Hits

Swiss-Prot: 75% identical to EXBB_PSEPU: Biopolymer transport protein ExbB (exbB) from Pseudomonas putida

KEGG orthology group: K03561, biopolymer transport protein ExbB (inferred from 100% identity to pfs:PFLU5999)

Predicted SEED Role

"MotA/TolQ/ExbB proton channel family protein" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K487 at UniProt or InterPro

Protein Sequence (322 amino acids)

>PFLU_RS29530 tonB-system energizer ExbB (Pseudomonas fluorescens SBW25)
MTRNTTPASPTKRHSPSRAWRAIAALLFSVLLAPTAAFADATAPATPPAAAAPAAPATAP
AATDPAQAADPASTDDTGVVLEEDNTLGMAHDLSPWGMYQNADVVVKAVMIGLAIASIIT
WTIWIAKGFELLGAKRRLRNEIVHLKKATTLKEASESATKKGTLANTLVHDALEEMRLSA
NAREKEGIKERVAFRLERLVAACGRNMSSGTGVLATIGSTAPFVGLFGTVWGIMNSFIGI
AKTQTTNLAVVAPGIAEALLATALGLVAAIPAVVIYNVFARSIAGYKAQVSDASAEVLLL
VSRDLDHLPTERSSQPHMVKVG