Protein Info for PFLU_RS28995 in Pseudomonas fluorescens SBW25

Annotation: NADPH:quinone oxidoreductase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08240: ADH_N
  • amino acids 28 to 87 (60 residues), 44.7 bits, E=1.6e-15
• PF00107: ADH_zinc_N
  • amino acids 151 to 269 (119 residues), 109.4 bits, E=1.9e-35
• PF13602: ADH_zinc_N_2
  • amino acids 184 to 322 (139 residues), 91 bits, E=2.1e-29

Best Hits

KEGG orthology group: K00344, NADPH2:quinone reductase [EC: 1.6.5.5] (inferred from 100% identity to pfs:PFLU5892)

MetaCyc: 36% identical to benzene hydroxylase alpha subunut (Geobacter metallireducens)
RXN-23213

Predicted SEED Role

"Quinone oxidoreductase (EC 1.6.5.5)" in subsystem ZZ gjo need homes (EC 1.6.5.5)

MetaCyc Pathways

• benzene degradation IV (anaerobic) (1/1 steps found)

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.5

Use Curated BLAST to search for 1.6.5.5

Sequence Analysis Tools

See C3K3Y2 at UniProt or InterPro

Protein Sequence (325 amino acids)

>PFLU_RS28995 NADPH:quinone oxidoreductase family protein (Pseudomonas fluorescens SBW25)
MKAVLCKAFGPAETLVLEEIASPAIKKNEILLDVHAAGVNFPDTLIIEGKYQFKPPFPFS
PGGEAAGVVSDVGEKVSHLKVGDRVMALTGWGSFAEQVAVPGYNVLPIPQSMDFNTAAAF
SMTYGTSMHALKQRANLQPGETLLVLGASGGVGLAAVEIGKAMGARVIAAASSADKLAVA
KAAGADELVNYSETSLKDEIKRLTDGNGADVIYDPVGGDLFDQAIRAIAWNGRLLAVGFA
SGRIPELPVNLALLKGAAVVGVFWGSFAQRQPQDNAANFQQLFTWYAEGKLKPLVSQVYP
LEQAAQAINDLGQRKAVGKVVVQIR