Protein Info for PFLU_RS28605 in Pseudomonas fluorescens SBW25

Annotation: putative 2-aminoethylphosphonate ABC transporter ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 TIGR03265: putative 2-aminoethylphosphonate ABC transporter, ATP-binding protein" amino acids 12 to 354 (343 residues), 519 bits, see alignment E=3.4e-160 PF00005: ABC_tran" amino acids 27 to 167 (141 residues), 118.7 bits, see alignment E=6.3e-38 PF08402: TOBE_2" amino acids 280 to 353 (74 residues), 28.5 bits, see alignment E=2.7e-10

Best Hits

KEGG orthology group: K02010, iron(III) transport system ATP-binding protein [EC: 3.6.3.30] (inferred from 100% identity to pfs:PFLU5811)

Predicted SEED Role

"Ferric iron ABC transporter, ATP-binding protein" in subsystem Iron acquisition in Vibrio or Transport of Iron

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.30

Use Curated BLAST to search for 3.6.3.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3Q4 at UniProt or InterPro

Protein Sequence (354 amino acids)

>PFLU_RS28605 putative 2-aminoethylphosphonate ABC transporter ATP-binding protein (Pseudomonas fluorescens SBW25)
MNTALTQPGAPMKVRGIQKRFGAFTALDNVSLDVAAGELVCLLGPSGCGKTTLLRCIAGL
ERQDSGELYLGNRDVSLLPPQARDYGILFQSYALFPNLTVEANIGYGLTGSGRDEVRQRV
GQMLELVGLLGSEKKYPGQLSGGQQQRVALARALAPAPSLLLLDEPMSALDARVREHLCT
ELRQLQRRLGITTLMVTHNQDEAMLMADRIAVMNNGKVEQYATPQEIYDRPATPFVAEFV
GQGNWLPFSRNSASHAQVGGMNMRLADDAGAAKSGRLFCRPEAISINPPVHEENLFPAKV
REITFLGNRCRMSFELEQLPGHPLLAELAPEAMPRLGAQDIWVALPPRSLQVFA