Protein Info for PFLU_RS28550 in Pseudomonas fluorescens SBW25

Annotation: RNA polymerase sigma factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 177 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 8 to 162 (155 residues), 82.6 bits, see alignment E=1.3e-27 PF04542: Sigma70_r2" amino acids 13 to 75 (63 residues), 34.6 bits, see alignment E=2e-12 PF08281: Sigma70_r4_2" amino acids 109 to 160 (52 residues), 56.5 bits, see alignment E=2.7e-19 PF04545: Sigma70_r4" amino acids 114 to 161 (48 residues), 28.1 bits, see alignment E=1.8e-10

Best Hits

KEGG orthology group: K03088, RNA polymerase sigma-70 factor, ECF subfamily (inferred from 100% identity to pfs:PFLU5800)

Predicted SEED Role

"FIG006045: Sigma factor, ECF subfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3P3 at UniProt or InterPro

Protein Sequence (177 amino acids)

>PFLU_RS28550 RNA polymerase sigma factor (Pseudomonas fluorescens SBW25)
MPSWNSQIARLFAEQKKALEAFVTRRTGSAQVAADLTQESFLRLARLDSGEKIDNLPAFL
FTIASNLVRDHQRQAIRRERLDAGEPSEELPCGNPGADEQLAAYQQEQLMQDAILALPEA
TRQIFLLYHVDELSYREIGERLSITPRSVEYQLRRALIECRAYIKTRLACETQGRRS