Protein Info for PFLU_RS28180 in Pseudomonas fluorescens SBW25

Annotation: DEAD/DEAH box helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 628 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF04851: ResIII" amino acids 23 to 195 (173 residues), 30.4 bits, see alignment E=5.4e-11 PF00270: DEAD" amino acids 25 to 199 (175 residues), 152.9 bits, see alignment E=1e-48 PF00271: Helicase_C" amino acids 236 to 344 (109 residues), 100.6 bits, see alignment E=9.2e-33

Best Hits

KEGG orthology group: K11927, ATP-dependent RNA helicase RhlE [EC: 3.6.4.13] (inferred from 100% identity to pfs:PFLU5724)

Predicted SEED Role

"ATP-dependent RNA helicase RhlE" in subsystem ATP-dependent RNA helicases, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3H0 at UniProt or InterPro

Protein Sequence (628 amino acids)

>PFLU_RS28180 DEAD/DEAH box helicase (Pseudomonas fluorescens SBW25)
MSFASLGLSEALVRAIEAAGYTEPTPVQQRAIPAVLQGRDLMVAAQTGTGKTGGFALPIL
ERLFPNGHPDKSQRHGPRQPRVLVLTPTRELAAQVHDSFKLYARDLKFVSACIFGGVGMN
PQVQAMSRGVDVLVACPGRLLDLCGQGSVDLSHVEILVLDEADRMLDMGFVHDVKKVLAR
LPAKRQNLLFSATFSQDITALAGKLLHNPERIEVTPPNTTVERIEQRVFRLAASHKRALL
AHLITAGAWEQVLVFTRTKHGANRLAEYLDKHGLTAVAIHGNKSQNARTKALADFKAGSV
RILVATDIAARGLDIDQLPHVVNFELPNVDEDYVHRIGRTGRAGRSGEAISLVAPDEEKL
LKSIERMTKQKIADGDLMGFDASAVEAEKPEVRERPDVRNPRNPRGPKGDGPNGGGGGGG
RRDKGKDKGGKEKAPTNGRGERPAREQKPREGTPAREQRQPSQPPRAAADRAPDEFLDDD
VDNFGNRVDYVPQAKPAQGRGRRPGAPAQGAGAGAPRGGQPQGARQNGPRNSSGGTTGTP
PAKRSGPRNGAPRDGQARREDSRSNNRRPARDDQPRLSEPAVQNPRGGPAPKIIHKESKA
DRFPTPEQLDQLPGRPRGEKPALLTRNR