Protein Info for PFLU_RS28105 in Pseudomonas fluorescens SBW25

Annotation: methionine adenosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01034: methionine adenosyltransferase
  • amino acids 6 to 393 (388 residues), 603.2 bits, E=9.1e-186
• PF00438: S-AdoMet_synt_N
  • amino acids 6 to 102 (97 residues), 142.9 bits, E=6e-46
• PF02772: S-AdoMet_synt_M
  • amino acids 115 to 232 (118 residues), 154.5 bits, E=2.1e-49
• PF02773: S-AdoMet_synt_C
  • amino acids 234 to 365 (132 residues), 221.9 bits, E=4e-70

Best Hits

Swiss-Prot: 100% identical to METK_PSEFS: S-adenosylmethionine synthase (metK) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00789, S-adenosylmethionine synthetase [EC: 2.5.1.6] (inferred from 100% identity to pfs:PFLU5709)

MetaCyc: 74% identical to methionine adenosyltransferase (Escherichia coli K-12 substr. MG1655)
Methionine adenosyltransferase. [EC: 2.5.1.6]

Predicted SEED Role

"S-adenosylmethionine synthetase (EC 2.5.1.6)" in subsystem Methionine Biosynthesis or Methionine Degradation or Quorum Sensing: Autoinducer-2 Synthesis (EC 2.5.1.6)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• S-adenosyl-L-methionine salvage II (3/3 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (4/5 steps found)
• S-adenosyl-L-methionine biosynthesis (1/1 steps found)
• L-methionine degradation I (to L-homocysteine) (2/3 steps found)
• S-adenosyl-L-methionine salvage I (2/4 steps found)
• ethene biosynthesis I (plants) (1/3 steps found)
• 2'-deoxymugineic acid phytosiderophore biosynthesis (1/4 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (3/7 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)
• L-methionine salvage cycle III (5/11 steps found)
• L-methionine salvage cycle I (bacteria and plants) (4/12 steps found)
• L-methionine salvage cycle II (plants) (3/11 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism
• Selenoamino acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.6

Sequence Analysis Tools

See C3K3F5 at UniProt or InterPro

Protein Sequence (396 amino acids)

>PFLU_RS28105 methionine adenosyltransferase (Pseudomonas fluorescens SBW25)
MSEYSLFTSESVSEGHPDKIADQISDAVLDAIIAEDKFARVACETLVKTGVAIIAGEVTT
TAWVDLEQIVRDVITDIGYTSSDVGFDGATCGVMNIIGKQSPDINQGVDRAKPEDQGAGD
QGLMFGYASNETDVLMPAPITFSHQLVQRQAEARKSGLLPWLRPDAKSQVTCRYEGGKVV
GIDAVVLSTQHNPDVSYADLREGVMELIVKHVLPAELLSKDTQFHINPTGQFIIGGPVGD
CGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLAERCEIQV
SYAIGVAQPTSISLNTFGTGKISDDKIVKLVREIFDLRPYAITTMLDLLHPMYQETAAYG
HFGRTPAQKTVGDDTFTTFTWEKTDRANDLRIAAGL