Protein Info for PFLU_RS28030 in Pseudomonas fluorescens SBW25

Annotation: TIGR01666 family membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 transmembrane" amino acids 21 to 38 (18 residues), see Phobius details amino acids 44 to 60 (17 residues), see Phobius details amino acids 68 to 85 (18 residues), see Phobius details amino acids 91 to 110 (20 residues), see Phobius details amino acids 116 to 134 (19 residues), see Phobius details amino acids 149 to 169 (21 residues), see Phobius details amino acids 396 to 416 (21 residues), see Phobius details amino acids 422 to 438 (17 residues), see Phobius details amino acids 445 to 463 (19 residues), see Phobius details amino acids 469 to 486 (18 residues), see Phobius details amino acids 492 to 510 (19 residues), see Phobius details amino acids 516 to 538 (23 residues), see Phobius details TIGR01667: integral membrane protein, YccS/YhfK family" amino acids 12 to 719 (708 residues), 933.1 bits, see alignment E=9.6e-285 TIGR01666: TIGR01666 family membrane protein" amino acids 14 to 717 (704 residues), 769.1 bits, see alignment E=3.8e-235 PF12805: FUSC-like" amino acids 71 to 355 (285 residues), 373.9 bits, see alignment E=6.7e-116 PF04632: FUSC" amino acids 399 to 705 (307 residues), 43 bits, see alignment E=4e-15 PF13515: FUSC_2" amino acids 415 to 530 (116 residues), 82.1 bits, see alignment E=5.8e-27

Best Hits

Swiss-Prot: 45% identical to YCCS_ECOLI: Inner membrane protein YccS (yccS) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU5693)

Predicted SEED Role

"FIG00956403: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3E0 at UniProt or InterPro

Protein Sequence (727 amino acids)

>PFLU_RS28030 TIGR01666 family membrane protein (Pseudomonas fluorescens SBW25)
MSSTSFKQSMRRLWALDKFSYSIRVFIALTGSMALCWYQDEMTLLIPLFLGIIASALAET
DDSWQGRLNALAVTLVCFSIAALSVELLFPYPWIFAISLALATFCLTMLGALGERYGAIA
SATLILSVYTMIGVDQRGGAVSDFWHEPLLLVAGAAWYGVLSVLWQALFSNQPVQQSLAR
LFRELGRYLKLKSSLFEPIRQLDVEARRLELAQQNGRVVAALNAAKEIILHRVGNGRPGS
KVSRYLKLYFLAQDIHERASSSHYPYNALAEAFFHSDVLFRCQRLLRQQGKACQALAESI
QLRQPFIYDDSFAEALGDLNASLEHLRIQSNPAWRGLLRSLRALAANLSTLDRLLGDASN
PDSLADATDSNLLDRAPRNLKEMWTRLRTQMTPTSLLFRHALRLSLALTVGYGMLHAIHA
SQGYWIILTTLFVCQPNYGATRRKLGQRIIGTAIGLTVAWALFDLFPSPLVQSMFAIAAG
LVFFINRTTRYTLATAAITLMVLFCFNQVGDGYGLFLPRLFDTLLGSLIAGLAVFLFLPD
WQGRRLNKVLANTLTCNSIYLRQIMQQYAAGKSDDLAYRLARRNAHNADAALSTTLANML
MEPGHFRKEADVGFRFLVLSHTLLSYLSGLGAHRETQLPAEVREHLIEGAGNTLAASIDD
IASGLANKQPIAIQSDAEEALAADLEQMPDEIDEGQRLVQTQLALICRQLGPLRTLAAHL
IKDTRAA