Protein Info for PFLU_RS27995 in Pseudomonas fluorescens SBW25

Annotation: choline dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 567 PF00732: GMC_oxred_N" amino acids 5 to 302 (298 residues), 197 bits, see alignment E=5.7e-62 TIGR01810: choline dehydrogenase" amino acids 6 to 539 (534 residues), 934.7 bits, see alignment E=7.7e-286 PF05199: GMC_oxred_C" amino acids 395 to 529 (135 residues), 141 bits, see alignment E=6.1e-45

Best Hits

Swiss-Prot: 100% identical to BETA_PSEFS: Oxygen-dependent choline dehydrogenase (betA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00108, choline dehydrogenase [EC: 1.1.99.1] (inferred from 100% identity to pfs:PFLU5686)

MetaCyc: 84% identical to choline dehydrogenase (Pseudomonas aeruginosa)
Choline dehydrogenase. [EC: 1.1.99.1]

Predicted SEED Role

"Choline dehydrogenase (EC 1.1.99.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.1.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.1

Use Curated BLAST to search for 1.1.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3D3 at UniProt or InterPro

Protein Sequence (567 amino acids)

>PFLU_RS27995 choline dehydrogenase (Pseudomonas fluorescens SBW25)
MSQEYDYIIVGAGSAGNTLATRLTEDEGVTVLLLEAGGPDYRLDFRTQMPAALAFPLQGR
RYNWAYETDPEPHMDGRRMECGRGKGLGGSSLINGMCYIRGNAMDYDNWAKLPGLENWTY
LDCLPYFRKAETRDIGPNDYHGGEGPVSVTTPKAGNNPLFHAMVEAGVQAGYPRTDDLNG
YQQEGFGPMDRTVTKNGRRASTARGYLDTAKKRSTLTIVTHALTDKVLFEGKRAVGVRYL
IGAAEERVEARARKEVLVCSGAIASPQLLQRSGVGPAKLLESLDIPVVHDLPGVGENLQD
HLELYLQYACTQPVSLYPSLLWYNQPAIGAEWLFNGTGIGASNQFEAGGFIRSRPEFDWP
NIQYHFLPVAINYNGSNGVKEHGFQAHMGSMRSPSRGRVQLKSKNPRDYPSILFNYMATE
QDWQEFRDGIRLTREIMQQPALDPYRGREISPGIDVQTDEQLDKFIREHAETAFHPSCSC
KMGTDEMAVVDGEGRVHGMQGLRVVDASIMPIITTGNLNAPTIMIAEKIADKIRGRQPLP
RSTADYYVAGDAPVRGKPLREVGPTAQ