Protein Info for PFLU_RS27880 in Pseudomonas fluorescens SBW25

Annotation: dimethylglycine demethylation protein DgcB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 652 transmembrane" amino acids 6 to 25 (20 residues), see Phobius details amino acids 68 to 87 (20 residues), see Phobius details amino acids 93 to 114 (22 residues), see Phobius details amino acids 135 to 153 (19 residues), see Phobius details amino acids 159 to 179 (21 residues), see Phobius details PF11982: DUF3483" amino acids 4 to 220 (217 residues), 279.2 bits, see alignment E=8.9e-87 PF13183: Fer4_8" amino acids 243 to 337 (95 residues), 36.3 bits, see alignment E=2.4e-12 PF13237: Fer4_10" amino acids 243 to 335 (93 residues), 37.8 bits, see alignment E=5.3e-13 PF13187: Fer4_9" amino acids 244 to 336 (93 residues), 30.6 bits, see alignment E=1e-10 PF02754: CCG" amino acids 404 to 482 (79 residues), 14.1 bits, see alignment E=1.4e-05 amino acids 526 to 612 (87 residues), 55.3 bits, see alignment E=2.1e-18

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU5663)

Predicted SEED Role

"DgcB Dimethylglycine demethylase subunit B"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3B0 at UniProt or InterPro

Protein Sequence (652 amino acids)

>PFLU_RS27880 dimethylglycine demethylation protein DgcB (Pseudomonas fluorescens SBW25)
MLNTLLPILLFAALGLAVLGALRRVNMWRRGRASKVDLLGGLLAMPKRYMVDLHHVVARD
KYIANTHVATALGFVLSALLAILVHGFGLHNRILGYALLLASVLMFVGATFVYLRRRNPP
SRLSKGPWMRLPKSLMAFSVSFFLVTLPVAGILPADFGGWLLAALLSVGVLWGVSEMFFG
MTWGGPMKHAFAGALHLAWHRRAERFGGGRSTGLKPLDLSDKSAPLGVEKPKDFTWNQLL
GFDACVQCGKCEAACPAFAAGQPLNPKKLIQDMVVGLAGGTDAKFAGSPYPSINGGGKPL
GEHGGNPHQPIVNGLVDAETLWSCTTCRACVEECPMMIEHVDAIVDMRRHLTLEKGATPN
KGAEVLENLIATDNPGGFAPGGRLNWAADLNLPLLSDKGSAEVLFWVGDGAFDMRNQRTL
RAFVKVLKAAKVDFAVLGLEERDSGDVARRLGDEATFQLLATRNIQTLAKYSFKRIVTCD
PHSFHVLKNEYGAFNGNYLVQHHSTFMAELIGEGALNLGQHKGNSVTYHDPCYLGRYNGE
YEAPRQVLRALGIEVKEMQRSGFRSRCCGGGGGAPITDIPGKQRIPDMRMDDIRETGAEL
VAVGCPQCTAMLEGVVEPRPLIKDIAELVADALLEDAAPAKAPAKLEPAEVH