Protein Info for PFLU_RS27865 in Pseudomonas fluorescens SBW25

Annotation: glycine-betaine demethylase subunit GbcA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 PF00355: Rieske" amino acids 55 to 131 (77 residues), 59.7 bits, see alignment E=2.1e-20 PF00848: Ring_hydroxyl_A" amino acids 189 to 412 (224 residues), 138.1 bits, see alignment E=3.9e-44

Best Hits

KEGG orthology group: K00479, Rieske 2Fe-2S family protein (inferred from 100% identity to pfs:PFLU5660)

MetaCyc: 87% identical to glycine betaine monooxygenase catalytic subunit (Pseudomonas aeruginosa)
RXN-22705 [EC: 1.14.13.251]

Predicted SEED Role

"GbcA Glycine betaine demethylase subunit A" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.13.251

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3A7 at UniProt or InterPro

Protein Sequence (431 amino acids)

>PFLU_RS27865 glycine-betaine demethylase subunit GbcA (Pseudomonas fluorescens SBW25)
MDVTATLSLGDPLEPARKATAQMLQERERTFSLPQPFYSDERLFDIDMQEIFQKEWLIAG
MTCEIPTKGNYLTLQVGKNPIIVIRGAEGVVHAFHNVCRHRGSRLCTSDKGKVAKLVCHY
HQWTYELDGRLLFAGTEMGADFDMKQYGLKPVNVKTAGGYIFISLAENPPAIDDFLSTLN
HYMEPYDMENTKVAIQTTLFEKANWKLVLENNRECYHCNASHPELLKTLLEWDDVTDPRA
DQAFKDHVAASAAAWDAEKIPYAHASFGLRNRIVRMPLLKGTVSMTLDGKQGCAKLMGRI
KNPDLGSMRILHLPHSWNHCMGDHIIVFTVWPISAQETMVTTKWIVHKDAVEGVDYDVER
MRQVWDATNDQDRRLAEENQRGINSTAYQPGPYSKTYEFGVVNFVDWYSERMLSNLGAAP
APYLKGVAVHE