Protein Info for PFLU_RS27850 in Pseudomonas fluorescens SBW25

Annotation: cytosine permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 481 transmembrane" amino acids 40 to 59 (20 residues), see Phobius details amino acids 65 to 85 (21 residues), see Phobius details amino acids 106 to 127 (22 residues), see Phobius details amino acids 146 to 165 (20 residues), see Phobius details amino acids 173 to 192 (20 residues), see Phobius details amino acids 204 to 225 (22 residues), see Phobius details amino acids 245 to 268 (24 residues), see Phobius details amino acids 288 to 312 (25 residues), see Phobius details amino acids 333 to 355 (23 residues), see Phobius details amino acids 361 to 382 (22 residues), see Phobius details amino acids 405 to 427 (23 residues), see Phobius details amino acids 433 to 455 (23 residues), see Phobius details PF02133: Transp_cyt_pur" amino acids 25 to 445 (421 residues), 180.3 bits, see alignment E=3e-57

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU5657)

Predicted SEED Role

"Cytosine/purine/uracil/thiamine/allantoin permease family protein" in subsystem Purine Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K3A4 at UniProt or InterPro

Protein Sequence (481 amino acids)

>PFLU_RS27850 cytosine permease (Pseudomonas fluorescens SBW25)
MNTPSDRSAAPTSTTLKIETRSIDYVPRNERHGKVWHQAPFWFTGNFVLTTMVTGFTGAA
LGLSLVYAVLAIVIGVCLGTFAMAFHANQGPRMGLPQMIQSRAQFGLRGAIVPFIAVVFV
YIGFNVFNVILATDAINTVLPGSRAPWYALMIILAVVIAVIGHDLLHTVQRWLTYVMISV
FAVLTVAAVTHLQADTAVADAHFSWSAFLIQLSAAAGYQISYAVYVSDYSRYLPHNTPSR
SVITWTYLGAAGSALWLMSLGAFLASALPSPDAIGSVQAVGNQLLPGFGTFTVLIAVPAL
VGIMAVNCYGAMLTGISAIDGFVPIKPRIKSRILGISLVAAIIFVIALCIPESYLSSFNT
FVLLMLYFLVPWTAVNLADFYAVRKGRYAISDIFNPAGIYGRWSSTGLVAYFLGLAAMVP
FMSLGFYQGPISFMLGGADIAFVVGLLVSALVYCVMSRQLDHAAESRAIKASEQALEGNV
S