Protein Info for PFLU_RS27620 in Pseudomonas fluorescens SBW25

Annotation: acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF12418: AcylCoA_DH_N
  • amino acids 3 to 35 (33 residues), 43.5 bits, see alignment (E = 6.5e-15)
• PF02771: Acyl-CoA_dh_N
  • amino acids 42 to 158 (117 residues), 41.8 bits, E=3.3e-14
• PF02770: Acyl-CoA_dh_M
  • amino acids 163 to 272 (110 residues), 53.2 bits, E=6.9e-18
• PF00441: Acyl-CoA_dh_1
  • amino acids 282 to 450 (169 residues), 66.8 bits, E=6.5e-22
• PF12806: Acyl-CoA_dh_C
  • amino acids 467 to 592 (126 residues), 129.5 bits, E=2.1e-41

Best Hits

KEGG orthology group: K00257, [EC: 1.3.99.-] (inferred from 100% identity to pfs:PFLU5609)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

• oleate β-oxidation (33/35 steps found)
• fatty acid salvage (6/6 steps found)
• adipate degradation (5/5 steps found)
• 2-methyl-branched fatty acid β-oxidation (11/14 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Brassinosteroid biosynthesis
• Caprolactam degradation
• Fatty acid metabolism
• Geraniol degradation
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.-

Use Curated BLAST to search for 1.3.8.7 or 1.3.99.-

Sequence Analysis Tools

See C3K358 at UniProt or InterPro

Protein Sequence (601 amino acids)

>PFLU_RS27620 acyl-CoA dehydrogenase (Pseudomonas fluorescens SBW25)
MPDYKAPLRDIRFVRDELLGYEAHYQSLPACQDATPDMVDAILEEGAKFCEQVLAPLNRV
GDIEGCTWSESGVKTPTGFKEAYKQFVEGGWPSLAHDVEHGGQGLPESLGLAVSEMVGAA
NWSWGMYPGLSHGAMNTISEHGTPEQQTAYLTKLVSGEWTGTMCLTEPHCGTDLGMLRTK
AEPQADGSYKVSGTKIFISAGEHDMADNIVHIVLARLPDAPAGTKGISLFIVPKFLPNAD
GSIGERNAVTCGSLEHKMGIHGNATCVMNFDAATGFLIGPANKGLNCMFTFMNTARLGTA
LQGLSHAEIGFQGGLKYARDRLQMRSLTGPKAPDKAADPIIVHPDVRRMLLTMKAFAEGN
RAMVYFTAKQVDIVKYGTDDEAKKHADGLLAFMTPIAKAFMTEVGFESANHGVQIYGGHG
FIAEWGMEQNVRDSRISMLYEGTTGIQALDLLGRKVLMTQGEALKGFTKIVHKFCQANEG
NEAVKEFVTPLAALNKEWGELTMKVGMAAMKDREEVGAASVDYLMYSGYACLAYFWADMA
RLAAEKLAAGTTEEAFYTAKLQTARFYFQRILPRTRTHVATMLSGANNLMDMKEEDFGLA
Y