Protein Info for PFLU_RS27560 in Pseudomonas fluorescens SBW25

Annotation: carbon-nitrogen hydrolase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 2 to 243 (242 residues), 206.3 bits, E=2.7e-65

Best Hits

Swiss-Prot: 84% identical to YPQQ_PSEPH: Hydrolase in pqqF 5'region from Pseudomonas protegens (strain DSM 19095 / LMG 27888 / CHA0)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU5596)

MetaCyc: 64% identical to 5-aminopentanamidase (Pseudomonas putida)
5-aminopentanamidase. [EC: 3.5.1.30]

Predicted SEED Role

"5-aminopentanamidase (EC 3.5.1.30)" in subsystem Lysine degradation (EC 3.5.1.30)

MetaCyc Pathways

• L-lysine degradation IV (5/5 steps found)
• superpathway of L-lysine degradation (17/43 steps found)

KEGG Metabolic Maps

• Lysine degradation

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.30

Use Curated BLAST to search for 3.5.1.30

Sequence Analysis Tools

See C3K345 at UniProt or InterPro

Protein Sequence (264 amino acids)

>PFLU_RS27560 carbon-nitrogen hydrolase family protein (Pseudomonas fluorescens SBW25)
MRVALYQCPPLPLDVAGNLKRLHQLAHEASDADVLVLPEMFLSGYNIGAEAVGALAEAQD
GPSAQAIGELAKSAGLAILYGYPERAEDGQIYNAVQLIDAHGQRLCNYRKTHLFGDLDHS
MFSAGDDDFPLVELNGWKLGFLICYDLEFPENTRRLALAGAELILVPTANMVPFDFVADV
TVRARAFENQCYVAYANYCGHEGEIQYCGQSSIAAPNGLRIAQAGLDEALIVGELDRQSI
LDARAANHYLQDRRPELYGALHKP