Protein Info for PFLU_RS27105 in Pseudomonas fluorescens SBW25

Annotation: 50S ribosomal protein L5

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 179 PF00281: Ribosomal_L5" amino acids 24 to 80 (57 residues), 92.3 bits, see alignment E=1.8e-30 PF00673: Ribosomal_L5_C" amino acids 84 to 177 (94 residues), 139.3 bits, see alignment E=3.6e-45

Best Hits

Swiss-Prot: 100% identical to RL5_PSEFS: 50S ribosomal protein L5 (rplE) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K02931, large subunit ribosomal protein L5 (inferred from 100% identity to pfs:PFLU5515)

MetaCyc: 68% identical to 50S ribosomal subunit protein L5 (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"LSU ribosomal protein L5p (L11e)" in subsystem Ribosome LSU bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K2W4 at UniProt or InterPro

Protein Sequence (179 amino acids)

>PFLU_RS27105 50S ribosomal protein L5 (Pseudomonas fluorescens SBW25)
MARLKEIYWKEIAPKLKEELKLSNVMEVPRVTKITLNMGLGEAVGDKKVIEHAVADLEKI
TGQKVVVTYARKSIAGFKVREGWPIGVKVTLRRERMYEFLDRLLSISLPRVRDFRGLNAK
SFDGRGNYSMGVKEQIIFPEIDYDKIDALRGLDITLTTTAKNDDEGRALLRAFKFPFRN