Protein Info for PFLU_RS27025 in Pseudomonas fluorescens SBW25

Annotation: excinuclease ABC subunit UvrA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 944 TIGR00630: excinuclease ABC subunit A" amino acids 3 to 923 (921 residues), 1486.6 bits, see alignment E=0 PF17760: UvrA_inter" amino acids 130 to 237 (108 residues), 135.5 bits, see alignment E=2.6e-43 PF17755: UvrA_DNA-bind" amino acids 290 to 398 (109 residues), 119.9 bits, see alignment E=2.6e-38

Best Hits

Swiss-Prot: 76% identical to UVRA_ECO57: UvrABC system protein A (uvrA) from Escherichia coli O157:H7

KEGG orthology group: K03701, excinuclease ABC subunit A (inferred from 75% identity to abo:ABO_0427)

MetaCyc: 76% identical to UvrABC excision nuclease subunit A (Escherichia coli K-12 substr. MG1655)
3.1.25.-

Predicted SEED Role

"Excinuclease ABC subunit A" in subsystem DNA repair, UvrABC system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K2U7 at UniProt or InterPro

Protein Sequence (944 amino acids)

>PFLU_RS27025 excinuclease ABC subunit UvrA (Pseudomonas fluorescens SBW25)
MDKILIRGARTHNLKNIDLTLPRDKLIVITGLSGSGKSSLAFDTLYAEGQRRYVESLSAY
ARQFLSMMEKPDVDTIEGLSPAISIEQKSTSHNPRSTVGTITEIYDYLRLLYARVGIPRC
PDHDIPLEAQTVSQMVDLVLAQPEGAKLMLLAPVIRERKGEHLSVFEELRAQGFVRARIN
GKLYELDEAPKLDKQKKHSIDVVVDRFKVRADLQQRLAESFETALKLADGIALVAPMDDE
PGEEIIFSARFACPICGHAISELEPKLFSFNNPAGACPTCDGLGVKQFFDIKRLVNGDLT
LAEGAIRGWDRRNVYYFQMLGSLASHYKFSLEVPFNQLPADQQKVILHGSGSQNVDFKYL
NDRGDIVKRSHPFEGIVPNLERRYRETESASVREELAKFLSTQPCPDCRGTRLRREARHV
WVGEKTLPAVTNLPIGDACEYFGVLKLTGRRGEIADKILKEIRERLQFLVNVGLDYLSLD
RSADTLSGGEAQRIRLASQIGAGLVGVLYILDEPSIGLHQRDNDRLLGTLKHLRDIGNTV
IVVEHDEDAIRLADYVVDIGPGAGVHGGHIVAEGTPAEVMAHPDSLTGKYLSGRVKIAVP
AKRTPRNKKLALHLKGARGNNLRNVDLEIPLGLLTCVTGVSGSGKSTLINNTLFPLSATA
LNGATTLEAAAHDSIKGLEHLDKVVDIDQSPIGRTPRSNPATYTGLFTPIRELFAGVPES
RSRGYGPGRFSFNVKGGRCEACQGDGLIKVEMHFLPDIYVPCDVCKSKRYNRETLEIKYK
GKSIHETLEMTIEEARVFFDAVPALARKLQTLMDVGLSYIKLGQSATTLSGGEAQRVKLS
RELSKRDTGKTLYILDEPTTGLHFADIQQLLDVLHRLRDHGNTVVVIEHNLDVIKTADWL
VDLGPEGGSKGGQIIAVGTPEQVSEMPQSHTGYYLKPLLARDRA