Protein Info for PFLU_RS27005 in Pseudomonas fluorescens SBW25

Annotation: trans-aconitate 2-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 253 PF13489: Methyltransf_23" amino acids 12 to 171 (160 residues), 55.6 bits, see alignment E=1.6e-18 PF05175: MTS" amino acids 22 to 104 (83 residues), 26.5 bits, see alignment E=1.4e-09 PF13847: Methyltransf_31" amino acids 34 to 143 (110 residues), 58.2 bits, see alignment E=2.6e-19 PF08241: Methyltransf_11" amino acids 35 to 126 (92 residues), 53.8 bits, see alignment E=7.5e-18 PF08242: Methyltransf_12" amino acids 35 to 125 (91 residues), 60 bits, see alignment E=9.4e-20 PF13649: Methyltransf_25" amino acids 35 to 123 (89 residues), 61.6 bits, see alignment E=2.8e-20

Best Hits

Swiss-Prot: 51% identical to TAM_ACIAC: Trans-aconitate 2-methyltransferase (tam) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K00598, trans-aconitate 2-methyltransferase [EC: 2.1.1.144] (inferred from 100% identity to pfs:PFLU5494)

Predicted SEED Role

"Trans-aconitate 2-methyltransferase (EC 2.1.1.144)" (EC 2.1.1.144)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.144

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K2U3 at UniProt or InterPro

Protein Sequence (253 amino acids)

>PFLU_RS27005 trans-aconitate 2-methyltransferase (Pseudomonas fluorescens SBW25)
MTWSAQQYTMFEQQRTRPVRDLVAAIPNAEVRHAVDLGCGPGNSTEVLAARFPHAHVTGM
DSSDDMLADARKRLPALNFKLADIGAWNPAQAFDVILANASLQWLPDHATLYPHLVKHLT
QGGTLAVQTPDNLDEPAHRLAREVAADGPWSAKIGAVKHNERHTASDYYELLSTHCSTVD
VWRTTYQHPLADHGAVVEWFKASALRPFLAPLSDEEKAAFLQAYKARITQAYPALADGTV
LLPFPRLFIIATR