Protein Info for PFLU_RS26900 in Pseudomonas fluorescens SBW25

Annotation: bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 378 PF00383: dCMP_cyt_deam_1" amino acids 12 to 109 (98 residues), 71.4 bits, see alignment E=7.7e-24 PF14437: MafB19-deam" amino acids 13 to 151 (139 residues), 52.8 bits, see alignment E=6e-18 TIGR00326: riboflavin biosynthesis protein RibD" amino acids 16 to 368 (353 residues), 391.7 bits, see alignment E=3e-121 TIGR00227: riboflavin-specific deaminase C-terminal domain" amino acids 155 to 370 (216 residues), 169.8 bits, see alignment E=6.1e-54 PF01872: RibD_C" amino acids 156 to 366 (211 residues), 162.5 bits, see alignment E=1.8e-51

Best Hits

KEGG orthology group: K11752, diaminohydroxyphosphoribosylaminopyrimidine deaminase / 5-amino-6-(5-phosphoribosylamino)uracil reductase [EC: 1.1.1.193 3.5.4.26] (inferred from 100% identity to pfs:PFLU5473)

Predicted SEED Role

"Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193)" in subsystem Riboflavin, FMN and FAD metabolism (EC 1.1.1.193, EC 3.5.4.26)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.26

Use Curated BLAST to search for 1.1.1.193 or 3.5.4.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K2S2 at UniProt or InterPro

Protein Sequence (378 amino acids)

>PFLU_RS26900 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD (Pseudomonas fluorescens SBW25)
MNSPSAEQAVLDAHYMARALELARKGLYTTHPNPRVGCVIVRDGQVVGEGWHVRTGEPHA
EVHALRAAGDKARGATAYVTLEPCSHYGHTPPCADALVSAGLARVVAAMQDPNPEVAGRG
MQRLAQAGIDVRSGVLESEARALNRGFLKRMEQGLPFVRVKLAMSLDGRTAMASGESQWI
TGPAARAAVQRLRAEASVVLTGADTVLADGARLTVRAAELGLDDATTALAMSRPPLRVLI
DGRLRVPLNAPFFKAGPALVITCVTAENQFPRGPECLVVPGVEGQVDLRSALVALAARGV
NDVLVEAGPSLAGAFAQQGLVDEYVIFVAGKFLGSAARPLLDWPLDKLADAPQLKITEMR
AVGDDWRVTAIPVPAASV