Protein Info for PFLU_RS26650 in Pseudomonas fluorescens SBW25

Annotation: rod shape-determining protein RodA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 381 transmembrane" amino acids 27 to 50 (24 residues), see Phobius details amino acids 56 to 77 (22 residues), see Phobius details amino acids 85 to 107 (23 residues), see Phobius details amino acids 121 to 140 (20 residues), see Phobius details amino acids 149 to 167 (19 residues), see Phobius details amino acids 172 to 190 (19 residues), see Phobius details amino acids 196 to 214 (19 residues), see Phobius details amino acids 278 to 305 (28 residues), see Phobius details amino acids 317 to 341 (25 residues), see Phobius details amino acids 350 to 371 (22 residues), see Phobius details TIGR02210: rod shape-determining protein RodA" amino acids 28 to 376 (349 residues), 437.1 bits, see alignment E=2.5e-135 PF01098: FTSW_RODA_SPOVE" amino acids 39 to 376 (338 residues), 369.5 bits, see alignment E=8.8e-115

Best Hits

Swiss-Prot: 53% identical to RODA_SHIFL: Peptidoglycan glycosyltransferase MrdB (mrdB) from Shigella flexneri

KEGG orthology group: K05837, rod shape determining protein RodA (inferred from 94% identity to pba:PSEBR_a4963)

MetaCyc: 53% identical to peptidoglycan glycosyltransferase MrdB (Escherichia coli K-12 substr. MG1655)
Peptidoglycan glycosyltransferase. [EC: 2.4.1.129]

Predicted SEED Role

"Rod shape-determining protein RodA" in subsystem Bacterial Cytoskeleton or Peptidoglycan Biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129

Use Curated BLAST to search for 2.4.1.129

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K2M4 at UniProt or InterPro

Protein Sequence (381 amino acids)

>PFLU_RS26650 rod shape-determining protein RodA (Pseudomonas fluorescens SBW25)
MKSNFDRILSSEDVMRRRATLLQRMHIDGPLLILLLTLAAGSLFVLYSASGKNWDLLIKQ
ASSFGLGLLSMVVIAQLEPRFMARWVPLGYVAGVLLLVVVDVMGHNAMGATRWINIPGVI
RFQPSEFMKILMPATIAWYLSKRTLPPQLKHVGISLILIGVPFILIVRQPDLGTSLLILA
GGAFVLFMGGLRWRWILSVLAAAVPVAVAMWFFFMHDYQKQRILTFLDPESDPLGTGWNI
IQSKAAIGSGGVFGKGWLLGTQSHLDFLPESHTDFIIAVLGEEFGLVGICALLLIYLLLI
GRGLVITAQAQTLFGKLLAGALTMTFFVYVFVNIGMVSGLLPVVGVPLPFISYGGTSLVT
LLSAFGVLMSIHTHRKWIAQV