Protein Info for PFLU_RS26515 in Pseudomonas fluorescens SBW25

Annotation: AMP nucleosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 TIGR01717: AMP nucleosidase" amino acids 12 to 487 (476 residues), 782.5 bits, see alignment E=7.9e-240 PF10423: AMNp_N" amino acids 13 to 169 (157 residues), 158.8 bits, see alignment E=8.7e-51 PF01048: PNP_UDP_1" amino acids 272 to 454 (183 residues), 70.3 bits, see alignment E=1.6e-23

Best Hits

KEGG orthology group: K01241, AMP nucleosidase [EC: 3.2.2.4] (inferred from 100% identity to pfs:PFLU5396)

Predicted SEED Role

"AMP nucleosidase (EC 3.2.2.4)" in subsystem Purine conversions (EC 3.2.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K2J8 at UniProt or InterPro

Protein Sequence (487 amino acids)

>PFLU_RS26515 AMP nucleosidase (Pseudomonas fluorescens SBW25)
MTEAFVVVQTAEEAVDRLAALHERATGALNQALKQYLKDRVEPDAAQRALFRYPELRLTY
HCHGEVPQTTRAYAKVQLPGTYSVTVTHPAAFRKYLLEQLVPLMHDFTVTVEVGVSQQNI
PYPYVVEQGDELAGSGVTAATLARVFPSTDLSAATDGIADGLYDWENTDPLPLALFDAAR
VDFSLRRLVHYTGSDWRHVQPWILLTNYHRYVDQFILHGLEQLRSDPRFIRMVLPGNVVI
DKAMDHGEASAIAAGVVWHRYQMPAYHLQATDGHGVTLVNIGVGPSNAKNITDHLAVLRP
HCWLMIGHCGGLRQSQTIGDYVLAHAYMRRDGILDRVVPPNIPIPALAEVQMALQQAAAN
VTGEKGEELKKRLRTGTVLTYDDRNWELRWAQERPLINLSRAVAVDMESGTIAAQGYRLR
VPYGTLLCVSDKPLHSEIKLPGSANAFYERAVSQHLKIGIEAVDLLRTELNSLHSRKLRS
FDEPPFR