Protein Info for PFLU_RS26195 in Pseudomonas fluorescens SBW25

Annotation: serine hydroxymethyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00464: SHMT
  • amino acids 9 to 385 (377 residues), 583.7 bits, E=1.5e-179
• PF00155: Aminotran_1_2
  • amino acids 64 to 370 (307 residues), 35.4 bits, E=7.1e-13

Best Hits

Swiss-Prot: 97% identical to GLYA1_PSE14: Serine hydroxymethyltransferase 1 (glyA1) from Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)

KEGG orthology group: K00600, glycine hydroxymethyltransferase [EC: 2.1.2.1] (inferred from 100% identity to pfs:PFLU5330)

MetaCyc: 74% identical to serine hydroxymethyltransferase (Escherichia coli K-12 substr. MG1655)
4.1.2.-; Glycine hydroxymethyltransferase. [EC: 2.1.2.1]; RXN-6321 [EC: 2.1.2.1]; RXN0-5240 [EC: 2.1.2.1]

Predicted SEED Role

"Serine hydroxymethyltransferase (EC 2.1.2.1)" in subsystem Folate Biosynthesis or Glycine Biosynthesis or Glycine and Serine Utilization or LMPTP YwlE cluster or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle or Serine Biosynthesis (EC 2.1.2.1)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• glycine betaine degradation III (7/7 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
• glycine degradation (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• glycine biosynthesis I (1/1 steps found)
• glycine betaine degradation I (6/8 steps found)
• folate transformations I (9/13 steps found)
• photorespiration II (6/10 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• photorespiration I (5/9 steps found)
• photorespiration III (5/9 steps found)
• glycine betaine degradation II (mammalian) (1/4 steps found)
• formaldehyde assimilation I (serine pathway) (6/13 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Glycine, serine and threonine metabolism
• Methane metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.1

Use Curated BLAST to search for 2.1.2.1

Sequence Analysis Tools

See C3K2D3 at UniProt or InterPro

Protein Sequence (417 amino acids)

>PFLU_RS26195 serine hydroxymethyltransferase (Pseudomonas fluorescens SBW25)
MFSRDLTIAKYDADLFAAMEQEAVRQEEHIELIASENYTSPAVMEAQGSVLTNKYAEGYP
GKRYYGGCEYVDVVEQLAIDRAKELFGADYANVQPHAGSQANSAVYLALLQGGDTILGMS
LAHGGHLTHGASVSSSGKLYNAVQYGIDANGLIDYDEVERLAVEHKPKMIVAGFSAYSQI
LDFPRFRAIADKVGAYLFVDMAHVAGLVAAGVYPNPVPYADVVTTTTHKTLRGPRGGLIL
ARANAEIEKKLNSAVFPGAQGGPLEHVIAAKAICFKEALQPEFKTYQQQVVKNAQTMASV
FIERGFDVVSGGTENHLFLLSLIKQDISGKDADAALGKAFITVNKNSVPNDPRSPFVTSG
LRFGTPAVTTRGFKEAECKELAGWICDILADLNNEAVIDAVREKVKAICKKLPVYGA