Protein Info for PFLU_RS26175 in Pseudomonas fluorescens SBW25

Annotation: NADP-specific glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02812: ELFV_dehydrog_N
  • amino acids 55 to 182 (128 residues), 174 bits, E=1.2e-55
• PF00208: ELFV_dehydrog
  • amino acids 200 to 443 (244 residues), 300.7 bits, E=8.9e-94

Best Hits

Swiss-Prot: 66% identical to DHE4_SALTY: NADP-specific glutamate dehydrogenase (gdhA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00262, glutamate dehydrogenase (NADP+) [EC: 1.4.1.4] (inferred from 100% identity to pfs:PFLU5326)

MetaCyc: 66% identical to glutamate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glutamate dehydrogenase (NADP(+)). [EC: 1.4.1.4]

Predicted SEED Role

"NADP-specific glutamate dehydrogenase (EC 1.4.1.4)" in subsystem Arginine and Ornithine Degradation or Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Proline Synthesis (EC 1.4.1.4)

MetaCyc Pathways

• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• L-glutamate biosynthesis III (1/1 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.4

Sequence Analysis Tools

See C3K2C9 at UniProt or InterPro

Protein Sequence (445 amino acids)

>PFLU_RS26175 NADP-specific glutamate dehydrogenase (Pseudomonas fluorescens SBW25)
MIESVESFLARLKKRDPDQPEFHQAVEEVLRSLWPFLEANPHYLTSGILERLCEPERAIT
FRVSWVDDHGKVQVNRGFRIQMNSAIGPYKGGLRFHPSVNLGVLKFLAFEQTFKNSLTSL
PMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADVDVPAGDIGVGAREIGFLFGQ
YKRLSNQFTSVLTGKGMTYGGSLIRPEATGFGCVYFAEEMLKRNGQRVEGKRVAVSGSGN
VAQYAARKVMDLGGKVISLSDSEGTLYAESGLTEEQWSALLALKNVQRGRISELAARYGL
EFRAGKTPWELACDIALPCATQNELDAEAARTLLRNGCVCVAEGANMPTTLEAVDIFIDA
GILFAPGKASNAGGVAVSGLEMSQNAMRLLWTAGEVDSKLHNIMQSIHHACVHYGEENGR
VNYVKGANIAGFVKVADAMLAQGIV