Protein Info for PFLU_RS25910 in Pseudomonas fluorescens SBW25

Annotation: DNA repair protein RecN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 557 TIGR00634: DNA repair protein RecN" amino acids 1 to 552 (552 residues), 542.1 bits, see alignment E=9.9e-167 PF13476: AAA_23" amino acids 5 to 223 (219 residues), 44 bits, see alignment E=4.2e-15 PF02463: SMC_N" amino acids 7 to 509 (503 residues), 57.3 bits, see alignment E=1.5e-19

Best Hits

Swiss-Prot: 45% identical to RECN_VIBC3: DNA repair protein RecN (recN) from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)

KEGG orthology group: K03631, DNA repair protein RecN (Recombination protein N) (inferred from 100% identity to pfs:PFLU5271)

Predicted SEED Role

"DNA repair protein RecN" in subsystem DNA-replication or DNA repair, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K277 at UniProt or InterPro

Protein Sequence (557 amino acids)

>PFLU_RS25910 DNA repair protein RecN (Pseudomonas fluorescens SBW25)
MLVHLSVHNYAIVEHLDLELDRGMSVITGETGAGKSIMLDALGLTLGDRADSGVVRPGAD
KADILATFDLADIPEAEAWLAERDLNNEGPCILRRVITAEGRSRGYINGTPCPLGDLKAL
GELLIDIHSQHEHQSLLKTDTHRRLLDEYAGATDLARQVQLAAQRWRQTRQELERLSNSG
DEQRARHQLLSYQLEELESLGLGETELEQLEQEHKNLTNAETLLGICRQVVEQCSESDSG
NVLNALTASLNRLSSVNNASGSLSEATTLLTSAQIQVEEAVGELNRFLDHFDADPARLQE
IEERLDTIYTLARKHRIQPTEVATMQQKLLDEIETLNANDESIERLGDELASFARHYQEK
ARELSDLRHQAATGLASAVELEIQRLGMPGGRFTIELHASTSNELQPYGLEQVELLVSAN
PGQPLKALAKVASGGELSRISLAIQVITAQTSRVPTLVFDEVDVGIGGPTAEIVGQLLRR
LGDRGQVLTVTHLPQVAAQGHQHLFVHKVRGSDATHTAVSKLNKSERVEEVARMLGGIDL
TKESLAHAKKMVVAAKA