Protein Info for PFLU_RS25670 in Pseudomonas fluorescens SBW25

Annotation: bifunctional aminoglycoside phosphotransferase/ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF01636: APH" amino acids 103 to 282 (180 residues), 30.8 bits, see alignment E=2.6e-11 PF13671: AAA_33" amino acids 338 to 483 (146 residues), 138.1 bits, see alignment E=2.7e-44

Best Hits

KEGG orthology group: K07028, (no description) (inferred from 100% identity to pfs:PFLU5224)

Predicted SEED Role

"FIG00955330: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K231 at UniProt or InterPro

Protein Sequence (518 amino acids)

>PFLU_RS25670 bifunctional aminoglycoside phosphotransferase/ATP-binding protein (Pseudomonas fluorescens SBW25)
MSQTLIAALQNPALYAHPVDAFQVIETHISWVVLTGPYAYKLKKPMNFGFLDFTDLEKRG
HFCNEELRLNQRLTEDLYLEVLPITGTAEAPQLGGDGPVIEYALKMRQFPQSQLLSTLQA
NGELTSAHIDEMAKQIAHFHLTAPKVPQEHPAGTPDEVMAPVRQNFDQIRPFLSDKADLA
QLDALQAWAESSFERLKPLFSQRKQEGFTRECHGDIHLGNATLIDGHVVIFDCIEFNEPF
RFTDVYADTGFLAMDLEDRGLKSLARRFISQYLELTGDYQGLEVLNFYKAYRALVRAKIA
LFSMPSEASPVQRATTLRQYRNYANLAESYSTIPSRFLAITHGVSAVGKSHVAMRLVEAL
GAVRLRSDVERKRLFGEQHVENTPQAGIYAADASVATYTRLNEIADTVLRAGYPVVLDAT
FLKREQRDAAAKVAEATGAPFLILDCNAPQAVIASWLAQRQADNNDPSDATLAVIEEQQA
NRDSLTAEELLLSKRVETNESGTLDALVAHIRQRLPGL