Protein Info for PFLU_RS25590 in Pseudomonas fluorescens SBW25

Annotation: exodeoxyribonuclease V subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1224 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR00609: exodeoxyribonuclease V, beta subunit" amino acids 8 to 1204 (1197 residues), 1088.5 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 9 to 159 (151 residues), 77.4 bits, see alignment E=3.8e-25 amino acids 334 to 445 (112 residues), 90.9 bits, see alignment E=3e-29 PF13245: AAA_19" amino acids 17 to 432 (416 residues), 42.1 bits, see alignment E=2.3e-14 PF13361: UvrD_C" amino acids 694 to 837 (144 residues), 58.4 bits, see alignment E=2.3e-19 PF12705: PDDEXK_1" amino acids 1096 to 1182 (87 residues), 24.7 bits, see alignment 5.6e-09

Best Hits

KEGG orthology group: K03582, exodeoxyribonuclease V beta subunit [EC: 3.1.11.5] (inferred from 81% identity to pfl:PFL_0741)

Predicted SEED Role

"Exodeoxyribonuclease V beta chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K220 at UniProt or InterPro

Protein Sequence (1224 amino acids)

>PFLU_RS25590 exodeoxyribonuclease V subunit beta (Pseudomonas fluorescens SBW25)
MTRKPLALAFPLKGSQLIEASAGTGKTFTISALYLRLVLGHGGDEAGFGRELLPPQILVV
TFTDAATKELRERIRIRLAEAARFFREEIEQPDALIADLRAEYPPEQWPACANRLDIAAQ
WMDEAAVSTIHSWCQRMLREHAFDSGSLFTQTLETDHSDLLGEVLRDYWRLFCYPMHDDA
LNWVRSNWGGPAALMPRVRALFGSERPTGETREPAELINACLQERREALQTLKAPWQQWA
AELRDICLQAVAAKTVDGRKMQARYFEPWFEKISAWAADETLEQLDIGTGFTRLTPDGMA
EAWKGEPPQHPGIDAMAGLKAALDALPTPDAAVLQHAAGWVGKRFEDEKRRRAEMGFDDM
LIRLNAALQADGGERLASVIREQFPVALIDEFQDTDPVQYSIFDSIYRIEENHLDSGLFL
IGDPKQAIYAFRGADIYTYLRARQSTTGRHHTLGTNFRSSHAMVEAVNHVFQRAETGRGA
FLFREPNGENPVPFHSVLSQGRKEQLQVNGQTLPAMNLWHLPTDQPVSNAVYRQQLAAAC
ATHIVELLNGGQQGTAGFLRPDVSFTGVLPSDIAILVRDGKEAQAVRTELAARGVRSVYL
SDKDSVFAAQEAHDLLAWLKACAEPDVERPLRAALACVTLNLSLPELERLNQDELAWESR
VMQFRGYRAIWRTQGVLPMLRRLLHDFKLPQTLIARSDGERVLTNLLHLSELLQQAASEL
DGEQALIRHLAEHLALSGQAGEEQILRLESDEQLVKVVTIHKSKGLEYDLVFLPFICSAK
PVDGSRLPLHYHDEHGKSHVSLRPTPELIAQADNERLAEDLRLLYVALTRAKHACWLGIA
DLKRGNSNSSVLHLSALGYLLGAGASLGESAGLARWLLDLQAGCPAIDYAQVPEAQPIVF
HPPRNEATLRAPLLPKRKAAENWWIASYSALRIGDSMSAATLEAPESPQAQKLFDDERLD
PDAPREVAASGGDIHRFPRGPNPGTFLHGLLEWAGEEGFNVTPEAIEKAVGARCNRRNWE
GWIITLSGWLGHLLQTPLPVDNGHVSLNGLQQYQIEMEFWFASHKVDVLALDKLVCQYTH
SGVSRVAAEPVLLNGMFKGFIDLTFEHDGRYYVADYKSNWLGPDDSAYTQDAMEQSILEH
RYDLQYVLYLLALHRQLKARLPDYDYDRHVGGALYLFLRGTRSASQGAYFTRPPRDLIEG
LDLLFQGKTLPPKVEPAWEQGVLL