Protein Info for PFLU_RS25585 in Pseudomonas fluorescens SBW25

Annotation: exodeoxyribonuclease V subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 701 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details TIGR01447: exodeoxyribonuclease V, alpha subunit" amino acids 24 to 681 (658 residues), 643.1 bits, see alignment E=2.3e-197 PF21185: RecD_N" amino acids 27 to 147 (121 residues), 38.3 bits, see alignment E=5.8e-13 PF13245: AAA_19" amino acids 196 to 348 (153 residues), 111.8 bits, see alignment E=1.3e-35 PF13604: AAA_30" amino acids 199 to 363 (165 residues), 92.5 bits, see alignment E=1.3e-29 PF13401: AAA_22" amino acids 207 to 329 (123 residues), 29.4 bits, see alignment E=4.2e-10 PF05127: Helicase_RecD" amino acids 210 to 329 (120 residues), 34 bits, see alignment E=1.2e-11 PF13538: UvrD_C_2" amino acids 609 to 655 (47 residues), 44.9 bits, see alignment 3.9e-15

Best Hits

KEGG orthology group: K03581, exodeoxyribonuclease V alpha subunit [EC: 3.1.11.5] (inferred from 100% identity to pfs:PFLU5212)

Predicted SEED Role

"Exodeoxyribonuclease V alpha chain (EC 3.1.11.5)" in subsystem DNA-replication (EC 3.1.11.5)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.5

Use Curated BLAST to search for 3.1.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K219 at UniProt or InterPro

Protein Sequence (701 amino acids)

>PFLU_RS25585 exodeoxyribonuclease V subunit alpha (Pseudomonas fluorescens SBW25)
MSLSPLPLEALMPLSRAADLVQLLERWVDRGWLRALDKAFVGFLHELDPHADPLVLLAAA
LTSHQLGHGHVCLDLFETLKAPDFALSLPPEGDLQTGAMLLPSQLLEGLDGAHWCHALAA
SHLVALAVDGSESAQSRPLVLSGKRLYLRRYWTYERRIDTALRLRLATQERVAADLPQRL
DSLFDQPPPDGVIDWQKLACALATRGAFSIVTGGPGTGKTTTVVRLLALLQAPSVESGSP
LRIRLAAPTGKAAARLTESISQQVLSLKVPDNVKEKIPTQVTTVHRLLGSRPGTRHFRHH
IGNPLPLDVLVVDEASMIDLEMMANLLDALPPHARLVLLGDKDQLASVEAGAVLGDLCRD
AEAGFYSAETRRWLQAVSGEDLSASGLQEDLDGTHPLAQQVVMLRYSRRFGEGSGIGQLA
RWVNQQNAEEARKLLAARSHDDLFCVSLKGEHDYALERLVLDGQGDGAEGYRYYLNLLQS
ARPSADTPREDTAWTHWAQQLLQAFDAFQLLCAVRKGPWGVEGLNLRITAALRKVRLIEG
DEQWYEGRPVLMTRNDYGLGLMNGDIGIALKLPESDGGPPVLRVAFPRNDGQGGVRFVLP
SRLNDVETVYAMTVHKSQGSEFTHTALILPDALNPVLTKELIYTGITRAKRWFSLIEPRG
GVFEEAVRRKVKRLSGLMLELGQESEISDAVLSLRHHPENT