Protein Info for PFLU_RS25575 in Pseudomonas fluorescens SBW25

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 420 transmembrane" amino acids 20 to 44 (25 residues), see Phobius details amino acids 149 to 171 (23 residues), see Phobius details PF17152: CHASE8" amino acids 41 to 141 (101 residues), 106.9 bits, see alignment E=8.6e-35 PF00672: HAMP" amino acids 177 to 228 (52 residues), 25.7 bits, see alignment 1.7e-09 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 241 to 399 (159 residues), 148 bits, see alignment E=1e-47 PF00990: GGDEF" amino acids 245 to 398 (154 residues), 158 bits, see alignment E=2.7e-50

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU5210)

Predicted SEED Role

"Inner membrane protein YfiN"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K217 at UniProt or InterPro

Protein Sequence (420 amino acids)

>PFLU_RS25575 diguanylate cyclase (Pseudomonas fluorescens SBW25)
MSGAKVRPTLRSVIGRGHLILALLAVTLASVSLTLLGVLALRVYAEHNLHLIARSINYTV
EAAVVFNDSAAATEALSLIASTEEVAQAEVLDANGKLLAQWIRPDTGLLSRVELELAHTL
LEQPISQPILHQGRTIGSIHLIGHGGSLLRFLLSGLAGIFICTALSAWIALHLARRLLRG
ITGPLQSLAAVAHAARSERDFDRRVPPAKIAELDSLGSDFNALLGEMEAWQSHLQSENES
LAHQANHDSLTGLPNRAFFEGRLIRALRSAAKAKEQVAVLYLDSDRFKEINDSFGHAAGD
AVLVAVAERVRAQLREDDLVARLGGDEFAILLAPLHKVEDAERIADKIIASMDLPISVPG
NTQVLTSLSIGIAIYPDHGATPGTLLNAADAAMYQAKRLSSGGQQTAESENPVVNVKHRS