Protein Info for PFLU_RS25540 in Pseudomonas fluorescens SBW25
Annotation: CoA-acylating methylmalonate-semialdehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 60% identical to MMSA_ANOGA: Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial (AGAP002499) from Anopheles gambiae
KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 100% identity to pfs:PFLU5203)MetaCyc: 72% identical to methylmalonate-semialdehyde dehydrogenase subunit (Burkholderia cepacia R34)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]
Predicted SEED Role
"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)
MetaCyc Pathways
- myo-inositol degradation I (6/7 steps found)
- β-alanine degradation II (2/2 steps found)
- propanoyl-CoA degradation II (4/5 steps found)
- L-valine degradation I (6/8 steps found)
- β-alanine degradation I (1/2 steps found)
- myo-, chiro- and scyllo-inositol degradation (6/10 steps found)
- 2,4-dinitrotoluene degradation (3/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.27
Use Curated BLAST to search for 1.2.1.27
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3K210 at UniProt or InterPro
Protein Sequence (505 amino acids)
>PFLU_RS25540 CoA-acylating methylmalonate-semialdehyde dehydrogenase (Pseudomonas fluorescens SBW25) MNASADISVQQVKLLINGEWVESKTTEWQDIVNPATQEVLAKVPFATADEVNAAIDAAHR AFQTWKLTPIGARMRIMLKLQALIREHSKRIAVVLSNEQGKTIADAEGDIFRGLEVVEHA CSIGTLQMGEFAENVAGGVDTYTLRQPIGVCAGITPFNFPAMIPLWMFPMAIACGNTFVL KPSEQDPLSTMLLVELALEAGVPAGVLNVVHGGKDVVDALCTHKDIKAVSFVGSTAVGTH VYDLAGKHGKRVQSMMGAKNHAVVLPDANREHTLNALVGAGFGAAGQRCMATSVVVLVGA SKQWLPDLKALAQKLKVNAGSEAGTDVGPVISKRAKARILELIESGVKEGAKLELDGRDI KVPGFEQGNFVGPTLFSGVTPSMQIYTQEIFGPVLVVLEVATLDEAIALVNANPFGNGTG LFTQSGAAARKFQSEIDVGQVGINIPIPVPVPFFSFTGSRGSKLGDLGPYGKQVVQFYTQ TKTVTSRWFDDDTVNDGVNTTINLR