Protein Info for PFLU_RS25215 in Pseudomonas fluorescens SBW25

Annotation: cytochrome o ubiquinol oxidase subunit I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 672 transmembrane" amino acids 16 to 38 (23 residues), see Phobius details amino acids 58 to 79 (22 residues), see Phobius details amino acids 103 to 127 (25 residues), see Phobius details amino acids 139 to 161 (23 residues), see Phobius details amino acids 189 to 213 (25 residues), see Phobius details amino acids 232 to 253 (22 residues), see Phobius details amino acids 285 to 305 (21 residues), see Phobius details amino acids 316 to 336 (21 residues), see Phobius details amino acids 346 to 369 (24 residues), see Phobius details amino acids 381 to 403 (23 residues), see Phobius details amino acids 415 to 440 (26 residues), see Phobius details amino acids 455 to 474 (20 residues), see Phobius details amino acids 494 to 517 (24 residues), see Phobius details amino acids 585 to 604 (20 residues), see Phobius details amino acids 610 to 627 (18 residues), see Phobius details TIGR02843: cytochrome o ubiquinol oxidase, subunit I" amino acids 2 to 647 (646 residues), 1121.6 bits, see alignment E=0 PF00115: COX1" amino acids 56 to 504 (449 residues), 466.9 bits, see alignment E=3.4e-144

Best Hits

Swiss-Prot: 80% identical to CYOB_PSEPU: Cytochrome bo(3) ubiquinol oxidase subunit 1 (cyoB) from Pseudomonas putida

KEGG orthology group: K02298, cytochrome o ubiquinol oxidase subunit I [EC: 1.10.3.-] (inferred from 100% identity to pfs:PFLU5139)

MetaCyc: 80% identical to cytochrome bo terminal oxidase subunit I (Pseudomonas putida KT2440)
RXN0-5268 [EC: 7.1.1.3]

Predicted SEED Role

"Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-)" in subsystem Terminal cytochrome O ubiquinol oxidase or Terminal cytochrome oxidases (EC 1.10.3.-)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.10.3.-

Use Curated BLAST to search for 1.10.3.- or 7.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K1U6 at UniProt or InterPro

Protein Sequence (672 amino acids)

>PFLU_RS25215 cytochrome o ubiquinol oxidase subunit I (Pseudomonas fluorescens SBW25)
MFGKLSWDAVPFHEPIVMITIAMIALGGLALFAAITYFKKWTYLWTEWLTSVDHKKIGVM
YVIVAMVMLLRGFADAIMMRTQLAMATEGSPGYLPPEHYDQIFTAHGVIMIIFMAMPFFT
GLMNLALPLQIGARDVAYPFLNSLSFWLLVSGVVLINVSLGVGEFAKTGWVAYPPLSGLQ
YSPGVGVDYYIWALQLSGLGTTLTGVNFLATVLKMRAPGMKLMDMPIFTWTCTWANVLIV
ASFPILAATMALLSLDRYLDFHIFTNELGGNPMMYVNLFWAWGHPEVYILILPAFGIFSE
VISTFTGKRLFGHHSMVYASGAISVLGFMVWLHHFFTMGSGASVNAFFGLATMLISIPTG
VKLFNWLFTIYHGRLRMTSQVLWTLGFMVTFAIGGMTGVLLAIPGADFVLHNSLFVIAHF
HNVIIGGAVFGYIAGFSFYFPKAFGFKLHEGWGKAAFWFWISGFFVAFMPLYALGFMGMT
RRLNATTNPEWVPYLYVAMFGAIMIAVGIACQLIQLYVSIRDRKQNACDSGDPWNGHTLE
WSTSSPPPFYNFAVIPTANTIDAFTEAKEDGTAYQRPKHYEPIHMPNNTATGVVMGALLT
VFGFAMIWHIWWLAIVGLVGTIGYFIVHAARDDQGYMVPVETIERIEAEQHARLVAEKKI
PANRVETSLEQA