Protein Info for PFLU_RS24835 in Pseudomonas fluorescens SBW25

Annotation: Fe-S protein assembly chaperone HscA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 620 TIGR01991: Fe-S protein assembly chaperone HscA" amino acids 21 to 617 (597 residues), 902.3 bits, see alignment E=7.5e-276 PF00012: HSP70" amino acids 21 to 601 (581 residues), 595.5 bits, see alignment E=9.9e-183 PF06723: MreB_Mbl" amino acids 127 to 377 (251 residues), 47.3 bits, see alignment E=1.4e-16

Best Hits

Swiss-Prot: 100% identical to HSCA_PSEFS: Chaperone protein HscA homolog (hscA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K04044, molecular chaperone HscA (inferred from 100% identity to pfs:PFLU5064)

MetaCyc: 65% identical to iron-sulfur cluster biosynthesis chaperone HscA (Escherichia coli K-12 substr. MG1655)
3.6.4.-

Predicted SEED Role

"Chaperone protein HscA" in subsystem Protein chaperones

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K1M1 at UniProt or InterPro

Protein Sequence (620 amino acids)

>PFLU_RS24835 Fe-S protein assembly chaperone HscA (Pseudomonas fluorescens SBW25)
MALLQIAEPGQSPQPHQRRLAVGIDLGTTNSLVAALRSGLSEPLPDADGQVILPSAVRYH
ADRTEVGESAKLAASADPLNTVLSVKRLMGRGLSDVKQLGDQLPYRFVGGESHMPFIDTV
QGPKSPVEVSADILKVLRQRAETTLGGELVGAVITVPAYFDDAQRQATKDAAKLAGLNVL
RLLNEPTAAAVAYGLDQHAEGLVAIYDLGGGTFDISILRLTGGVFEVLATGGDSALGGDD
FDHTVAGWIISSAGLSADLDPGAQRNLLQTACAAKEALTDAATVEVSYGTWSAQLTREAF
DALIEPMVARSLKACRRAVRDSGVELEDVGAVVMVGGSTRVPRVRDAVAEAFGRQPLTEI
DPDQVVAIGAAIQADTLAGNKRDGGELLLLDVIPLSLGLETMGGLMEKVIPRNTTIPVAR
AQDFTTYKDGQTAMMIHVLQGERELISDCRSLARFELRGIPAMVAGAAKIRVTYQVDADG
LLSVAARELASGVEASIQVKPSYGLTDGEIAKMLKDSFQYAGDDKVARVLREQQVDAQRL
LEAVQGALDVDGERLLDAEERMVIDLQMQELAELIKGTDGYAIEQQTKRLSQVTDAFAAR
RMDQSVKAALAGRNLNELEE