Protein Info for PFLU_RS24810 in Pseudomonas fluorescens SBW25

Annotation: type IV pilus biogenesis/stability protein PilW

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 252 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR02521: type IV pilus biogenesis/stability protein PilW" amino acids 8 to 240 (233 residues), 248 bits, see alignment E=4.5e-78 PF13181: TPR_8" amino acids 39 to 69 (31 residues), 14.4 bits, see alignment 1.8e-05 amino acids 106 to 131 (26 residues), 12.9 bits, see alignment (E = 5.5e-05) PF13432: TPR_16" amino acids 76 to 131 (56 residues), 28.5 bits, see alignment E=8.8e-10 amino acids 152 to 200 (49 residues), 18 bits, see alignment 1.7e-06

Best Hits

KEGG orthology group: K02656, type IV pilus assembly protein PilF (inferred from 100% identity to pfs:PFLU5059)

Predicted SEED Role

"Type IV pilus biogenesis protein PilF"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K1L6 at UniProt or InterPro

Protein Sequence (252 amino acids)

>PFLU_RS24810 type IV pilus biogenesis/stability protein PilW (Pseudomonas fluorescens SBW25)
MPLRLALLLLVTGLAAGCVSSGHDSPLQTGKGRDEARVAYVQLGLGYLRQGMSEQAKVPL
KKALELDSDDADANAALALVFQAQAEPELAEQYFHKALASRPADPRLLNNYGSFLFEQKR
YDQAALYFQQASADTLYPERSRVFENLGVTSMRLGQRDSARRQLEKALHLNGRQPRALLE
MAELSYEDRHYVPARDYYERFSLLSGQNARSLLLGVRLATVHEERDTAARFGQQLERLYP
GTPEYQQYLSEQ