Protein Info for PFLU_RS24715 in Pseudomonas fluorescens SBW25

Annotation: PTS system trehalose-specific EIIBC component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 480 transmembrane" amino acids 112 to 132 (21 residues), see Phobius details amino acids 157 to 177 (21 residues), see Phobius details amino acids 189 to 207 (19 residues), see Phobius details amino acids 237 to 254 (18 residues), see Phobius details amino acids 266 to 288 (23 residues), see Phobius details amino acids 294 to 336 (43 residues), see Phobius details amino acids 348 to 369 (22 residues), see Phobius details amino acids 405 to 424 (20 residues), see Phobius details amino acids 444 to 468 (25 residues), see Phobius details TIGR01992: PTS system, trehalose-specific IIBC component" amino acids 7 to 469 (463 residues), 718.8 bits, see alignment E=3e-220 PF00367: PTS_EIIB" amino acids 10 to 43 (34 residues), 51 bits, see alignment (E = 7.6e-18) TIGR00826: PTS system, glucose-like IIB component" amino acids 30 to 113 (84 residues), 48.3 bits, see alignment E=1e-16 PF02378: PTS_EIIC" amino acids 112 to 411 (300 residues), 161 bits, see alignment E=3.8e-51

Best Hits

Swiss-Prot: 53% identical to PTTBC_BACSU: PTS system trehalose-specific EIIBC component (treP) from Bacillus subtilis (strain 168)

KEGG orthology group: K02818, PTS system, trehalose-specific IIB component [EC: 2.7.1.69] K02819, PTS system, trehalose-specific IIC component (inferred from 100% identity to pfs:PFLU5040)

MetaCyc: 48% identical to Enzyme IIscr (Staphylococcus xylosus)
SUCROSEPHOSPHO-RXN [EC: 2.7.1.211]

Predicted SEED Role

"PTS system, trehalose-specific IIB component (EC 2.7.1.69) / PTS system, trehalose-specific IIC component (EC 2.7.1.69)" in subsystem Trehalose Uptake and Utilization (EC 2.7.1.69)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.211 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K1J7 at UniProt or InterPro

Protein Sequence (480 amino acids)

>PFLU_RS24715 PTS system trehalose-specific EIIBC component (Pseudomonas fluorescens SBW25)
MSHDYSNIAREILENLGGSDNLEQAAHCVTRLRLALKDPSLVNSSALNQVDLVKGSFFTG
GLFQVVIGPGEVEKVYAALREQTGLAAATIADVKKKGADKTNAMQRLVRVFSDVFMPILP
ALIIAGLLMGVNNLMGAKGMFIEGKTLLEAYPNLDGLWSLINLMANTSFVFLPALVGWSA
AKRFGGSEILGIVLGLMLVHPDLLNAWNYGKAVAGLDGQSLPYFDIFGWFQIEKVGYQGQ
ILPILMAAYVMSVIEKWLRARVPNAIQLLVVPITTIVVTGVLALAIIGPVTRHLGILITE
GVVTLFDLAPMVGGAIFGLLYAPLVITGMHHMFLAVDLQLISTQGGTFIWPMIVMSNLAQ
GSAALAVFYTTRNARDKSMASTSAISAYFGITEPAMFGVNLRFKFPFYAALVGSALGSIF
LSLNKVQASAIGVGGLPGFISIVPQSIAVFVIGMVIAMVVPFVLTCALSMKIVRPGYRVA