Protein Info for PFLU_RS24710 in Pseudomonas fluorescens SBW25

Annotation: alpha,alpha-phosphotrehalase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 548 TIGR02403: alpha,alpha-phosphotrehalase" amino acids 3 to 545 (543 residues), 861.4 bits, see alignment E=1.4e-263 PF00128: Alpha-amylase" amino acids 26 to 363 (338 residues), 413.4 bits, see alignment E=1.4e-127 PF11941: DUF3459" amino acids 450 to 541 (92 residues), 26 bits, see alignment E=1.5e-09 PF16657: Malt_amylase_C" amino acids 467 to 542 (76 residues), 28.9 bits, see alignment E=1.4e-10

Best Hits

Swiss-Prot: 58% identical to TREC_BACSU: Trehalose-6-phosphate hydrolase (treA) from Bacillus subtilis (strain 168)

KEGG orthology group: K01226, trehalose-6-phosphate hydrolase [EC: 3.2.1.93] (inferred from 100% identity to pfs:PFLU5039)

MetaCyc: 58% identical to trehalose-6-phosphate hydrolase (Bacillus subtilis)
Alpha,alpha-phosphotrehalase. [EC: 3.2.1.93]

Predicted SEED Role

"Trehalose-6-phosphate hydrolase (EC 3.2.1.93)" in subsystem Trehalose Uptake and Utilization (EC 3.2.1.93)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.93

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K1J6 at UniProt or InterPro

Protein Sequence (548 amino acids)

>PFLU_RS24710 alpha,alpha-phosphotrehalase (Pseudomonas fluorescens SBW25)
MQNWQHSVIYQIYPKSFHSHAGNATGDLLGIVDKLDYLKWLGVDCLWITPFLRSPQRDNG
YDISDYYAIDPSYGTMADCDLLISEAAKRGIKLMLDIVVNHTSIEHEWFQQARSSLDNPY
RDFYIWRDQPNNWESKFGGSAWEYEAQTGQYFLHLFDHTQADLNWDNPKVRAEVFKLMRF
WRDKGVGGFRLDVINLISKPADFPEDSSDGRRFYTDGPNVHDYLQEMHREVFEGHDLINV
GEMSSTSLEHCIRYSRPESKELSMTFNFHHLKVDYPNLQKWVKADFDFLQLKQILSDWQL
GMQAGGGWNALFWCNHDQPRVVSRFGHDGEHRVVSAKMLATALHFLQGTPFVYQGEELGM
TNPGFDRIEQYRDVETLNIFRLKRDAGESEASSMAAIMQKSRDNGRTPMQWNAQANAGFS
SGEPWIGIPANAAQINVERQLDDPDSVLHHYRALIALRRHEPLIQAGVYRQLLQDHLQVW
AYLREGHGEQLLVLNNFYGTPCEIQLPDGVINPASEQRLLISNYPDCPVRTGTVALRPYE
SFVLHLKD