Protein Info for PFLU_RS24600 in Pseudomonas fluorescens SBW25

Annotation: MHS family MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 transmembrane" amino acids 35 to 51 (17 residues), see Phobius details amino acids 59 to 81 (23 residues), see Phobius details amino acids 93 to 113 (21 residues), see Phobius details amino acids 119 to 142 (24 residues), see Phobius details amino acids 158 to 182 (25 residues), see Phobius details amino acids 193 to 212 (20 residues), see Phobius details amino acids 244 to 264 (21 residues), see Phobius details amino acids 283 to 304 (22 residues), see Phobius details amino acids 313 to 332 (20 residues), see Phobius details amino acids 338 to 360 (23 residues), see Phobius details amino acids 376 to 394 (19 residues), see Phobius details amino acids 405 to 424 (20 residues), see Phobius details PF00083: Sugar_tr" amino acids 22 to 228 (207 residues), 92.3 bits, see alignment E=3.4e-30 amino acids 257 to 435 (179 residues), 45.9 bits, see alignment E=4e-16 PF07690: MFS_1" amino acids 29 to 292 (264 residues), 52.7 bits, see alignment E=3.3e-18 amino acids 283 to 428 (146 residues), 47.7 bits, see alignment E=1.1e-16

Best Hits

Swiss-Prot: 55% identical to YHJE_ECOLI: Inner membrane metabolite transport protein YhjE (yhjE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU5016)

Predicted SEED Role

"Permeases of the major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K1H5 at UniProt or InterPro

Protein Sequence (436 amino acids)

>PFLU_RS24600 MHS family MFS transporter (Pseudomonas fluorescens SBW25)
MSTTYNEAATAAPTNSTARVATASIVGTAIEFYDFYIYATAAALVIGPVFFPQTSGTAQM
LASFLTFGIAFIARPLGSALFGHFGDRIGRKSTLVASLLLMGVCTTLIGLLPGYDSIGAW
APILLCILRFGQGLGLGGEWGGAALLATENAPKGKRAWFGMFPQLGPSIGFLAANGLFLI
LAMSLNDEQFRSWGWRIPFILSAALVMVGLYARLKLHETPVFANAVAKEAPVKVPLVELF
SQHWLPVLLGAASMVVCYALFYITTAFSLSYGVSTLGYSRETFLGLLCFAVLFMGLATPL
AALASDRYGRRPVLIVGAILAILSGFTMEPLLTHGSTWAVALFLALELFLMGVTFAPMGA
MLPELFPTRVRYTGASAAYNLGGIVGASAAPFFATKLVAMGGLSYVGGYVSAAALLSLIA
VLCLKETRDNDLNKVA