Protein Info for PFLU_RS24170 in Pseudomonas fluorescens SBW25

Annotation: Nudix family hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 314 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details TIGR00586: mutator mutT protein" amino acids 1 to 125 (125 residues), 116 bits, see alignment E=5.7e-38 PF00293: NUDIX" amino acids 3 to 123 (121 residues), 70.5 bits, see alignment E=2.1e-23 PF14815: NUDIX_4" amino acids 8 to 124 (117 residues), 90.3 bits, see alignment E=1.1e-29 PF02581: TMP-TENI" amino acids 132 to 309 (178 residues), 129.3 bits, see alignment E=1.6e-41

Best Hits

KEGG orthology group: K03574, 7,8-dihydro-8-oxoguanine triphosphatase [EC: 3.6.1.-] (inferred from 100% identity to pfs:PFLU4931)

Predicted SEED Role

"Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-) / Thiamin-phosphate pyrophosphorylase-like protein" in subsystem Nudix proteins (nucleoside triphosphate hydrolases) (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-

Use Curated BLAST to search for 3.6.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JYU5 at UniProt or InterPro

Protein Sequence (314 amino acids)

>PFLU_RS24170 Nudix family hydrolase (Pseudomonas fluorescens SBW25)
MKRVHVAAAVIRGVDGRILLARRADTQHQGGLWEFPGGKVEADESVASALSRELQEELGI
QVTTARPLIKVQHDYPDKQVLLDVWEVSAFTGEPQGVEGQPLEWVAPRDLINYEFPAANA
PIVAAARLPAEYLITPDELETPALLRGIQKAIAGGIKLVQLRAPNGYDPKYRDLAVDAVG
LCAGKAQLMLKGPFEWLGDFPAAGWHMTAAQLRKYASKGRPLPKDRWLAASCHNAEELAL
AQMMDVDFVTLSPVQPTQTHPDAQPLGWAQAAELIRGFSKPVFLLGGVGPAEREQAWEAG
AQGVAGIRAFWPQT