Protein Info for PFLU_RS23595 in Pseudomonas fluorescens SBW25

Annotation: LacI family DNA-binding transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 PF00356: LacI" amino acids 16 to 61 (46 residues), 49.2 bits, see alignment 7.1e-17 PF00532: Peripla_BP_1" amino acids 73 to 320 (248 residues), 89.6 bits, see alignment E=5.2e-29 PF13407: Peripla_BP_4" amino acids 75 to 288 (214 residues), 41.3 bits, see alignment E=2.8e-14 PF13377: Peripla_BP_3" amino acids 182 to 342 (161 residues), 107.2 bits, see alignment E=2e-34

Best Hits

KEGG orthology group: K06145, LacI family transcriptional regulator, gluconate utilization system Gnt-I transcriptional repressor (inferred from 100% identity to pfs:PFLU4812)

Predicted SEED Role

"Gluconate utilization system Gnt-I transcriptional repressor" in subsystem D-gluconate and ketogluconates metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JZ46 at UniProt or InterPro

Protein Sequence (342 amino acids)

>PFLU_RS23595 LacI family DNA-binding transcriptional regulator (Pseudomonas fluorescens SBW25)
MTSKNDKKMRTTGRPTLNEVARLAGVSPITASRALRGISTVATELVEKVQHAAAELNYVV
NPAARALASAQSHSVVVLVPSLSNLLFIETLEAIHQVLRPKGFEVLIGNSHYSRDEEENL
LRNYMAYQPRGLLLTGFDRTESARRMVEASNVPCVYMMDLDPNAGVNCVGFSQLAAGETA
AAHLISRGRKRLAYIGAQLDQRTLLRGEGFRRALQQAGLYDPALELLTPRPSSVGLGGEL
FLQLLAAHPDVDAIFFGNDDLAQGALLEALRHGIKVPERVAVLGFNDLPASSFMVPRLSS
ISTPREAIGRRAAEHLLTIMAGNKIAKPVVDMGFELQVREST