Protein Info for PFLU_RS23315 in Pseudomonas fluorescens SBW25
Annotation: aspartate aminotransferase family protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 85% identical to ARGD1_PSESM: Acetylornithine aminotransferase 1 (argD1) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
KEGG orthology group: K00821, acetylornithine/N-succinyldiaminopimelate aminotransferase [EC: 2.6.1.11 2.6.1.17] (inferred from 100% identity to pfs:PFLU4758)MetaCyc: 80% identical to succinylornithine transaminase subunit (Pseudomonas aeruginosa)
Succinylornithine transaminase. [EC: 2.6.1.81]; Ornithine aminotransferase. [EC: 2.6.1.81, 2.6.1.13]; Acetylornithine transaminase. [EC: 2.6.1.81, 2.6.1.13, 2.6.1.11]
Predicted SEED Role
"Succinylornithine transaminase (EC 2.6.1.81)" in subsystem Arginine and Ornithine Degradation (EC 2.6.1.81)
MetaCyc Pathways
- aspartate superpathway (22/25 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- superpathway of arginine and polyamine biosynthesis (15/17 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- L-lysine biosynthesis I (9/9 steps found)
- L-citrulline biosynthesis (8/8 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
- L-arginine degradation II (AST pathway) (5/5 steps found)
- L-arginine degradation XIII (reductive Stickland reaction) (5/5 steps found)
- L-ornithine biosynthesis I (5/5 steps found)
- superpathway of L-citrulline metabolism (10/12 steps found)
- L-arginine degradation VI (arginase 2 pathway) (4/4 steps found)
- L-Nδ-acetylornithine biosynthesis (6/7 steps found)
- L-arginine degradation I (arginase pathway) (3/3 steps found)
- L-ornithine biosynthesis II (3/3 steps found)
- L-proline biosynthesis III (from L-ornithine) (3/3 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Lysine biosynthesis
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.11, 2.6.1.13, 2.6.1.17, 2.6.1.81
Use Curated BLAST to search for 2.6.1.11 or 2.6.1.13 or 2.6.1.17 or 2.6.1.81
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3JXZ0 at UniProt or InterPro
Protein Sequence (406 amino acids)
>PFLU_RS23315 aspartate aminotransferase family protein (Pseudomonas fluorescens SBW25) MSVEQAPVQRADFDQVMVPNYAPAAFIPVRGAGSRVWDQAGRELIDFAGGIAVNVLGHAH PALVGALTEQANKLWHVSNVFTNEPALRLAHKLIDATFAERVFFCNSGAEANEAAFKLAR RVAFDRFGTEKYEIIAALNSFHGRTLFTVNVGGQSKYSDGFGPKITGITHVPYNDLDALK AAVSDKTCAVVLEPIQGEGGVLPAELAYLQGARELCDANNALLVFDEVQTGMGRSGHLFA YQHYGVVPDILTSAKSLGGGFPIAAMLTTEALAKHLVVGTHGTTYGGNPLACAVAEAVID VINTPEVLAGVNAKHDLFKARLEQIGKQYGIFTEVRGMGLLIGCVLSDAFKGKAKDVFNA AEKENLMILQAGPDVVRFAPSLVVEEADINEGLDRFERAVKSLTHA