Protein Info for PFLU_RS23300 in Pseudomonas fluorescens SBW25

Annotation: succinylglutamate-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 488 TIGR03240: succinylglutamate-semialdehyde dehydrogenase" amino acids 4 to 486 (483 residues), 833 bits, see alignment E=3.7e-255 PF00171: Aldedh" amino acids 10 to 461 (452 residues), 408.4 bits, see alignment E=1.7e-126

Best Hits

Swiss-Prot: 100% identical to ASTD_PSEFS: N-succinylglutamate 5-semialdehyde dehydrogenase (astD) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K06447, succinylglutamic semialdehyde dehydrogenase [EC: 1.2.1.71] (inferred from 100% identity to pfs:PFLU4754)

MetaCyc: 61% identical to aldehyde dehydrogenase (Escherichia coli K-12 substr. MG1655)
Succinylglutamate-semialdehyde dehydrogenase. [EC: 1.2.1.71]

Predicted SEED Role

"Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71)" in subsystem Arginine and Ornithine Degradation (EC 1.2.1.71)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.71

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3JXY7 at UniProt or InterPro

Protein Sequence (488 amino acids)

>PFLU_RS23300 succinylglutamate-semialdehyde dehydrogenase (Pseudomonas fluorescens SBW25)
MNSLYIAGSWLAGQGELFESRNPVTQHVLWAGNGATAEQVESAVQAARQAFPAWARRPLE
ERISVLETFAATLKNRADEIARCIGEETGKPLWESVTEVTSMANKIAISVQSYRERTGEK
SGPLGDATAVLRHKPHGVVAVFGPYNFPGHLPNGHIVPALLAGNTVLFKPSELTPKVAEL
TVQCWIEAGLPAGVLNLLQGARETGIALAANPGIDGLFFTGSSRTGNHLHQQFSGRPDKI
LALEMGGNNPLVVDEVADVDAAVYTIIQSAFISAGQRCTCARRLLVPEGAWGDALLARLV
AVSATIAVGAFDQQPAPFMGSVISLAAAKALMDAQELMLANGAVALLEMTQPQDQAALLT
PGIIDVTGVTAREDEELFGPLLQVIRYADFAAAIAEANNTQYGLAAGLLSDSEARYQQFW
LESRAGIVNWNKQLTGAASTAPFGGVGASGNHRASAYYAADYCAYPVASLETPSLVVPAT
LTPGVRLS