Protein Info for PFLU_RS23170 in Pseudomonas fluorescens SBW25

Annotation: flagellar hook assembly protein FlgD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 241 PF03963: FlgD" amino acids 19 to 90 (72 residues), 80.3 bits, see alignment E=1.4e-26 PF13861: FLgD_tudor" amino acids 99 to 237 (139 residues), 58.1 bits, see alignment E=1.3e-19 PF13860: FlgD_ig" amino acids 123 to 193 (71 residues), 76.3 bits, see alignment E=2.2e-25

Best Hits

KEGG orthology group: K02389, flagellar basal-body rod modification protein FlgD (inferred from 100% identity to pfs:PFLU4729)

Predicted SEED Role

"Flagellar basal-body rod modification protein FlgD" in subsystem Flagellar motility or Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K0Q8 at UniProt or InterPro

Protein Sequence (241 amino acids)

>PFLU_RS23170 flagellar hook assembly protein FlgD (Pseudomonas fluorescens SBW25)
MAIVDTSNNTAVQDLFNSKVKTATDNSSVGDAAKSATGNQSLGKDAFLQLLVTQLKNQNP
LSPQDNGAFVAQLAQFSSLEGINTLNDSVNNISSNFSSSQALQASSLVGRSIIIQTDKAM
VDTSKSMNGSVAVTSAVGNVSVKITDKDGTVVRTLDMGAQSAGTQSFIWDGKNENGEVAP
AGTYTFNATTKNDKGEAVALATSLPATVTSVTLSKTGGEMLLNLAGGMGSVKLSQIQTIG
T