Protein Info for PFLU_RS23125 in Pseudomonas fluorescens SBW25

Annotation: HAMP domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 166 to 188 (23 residues), see Phobius details PF00512: HisKA" amino acids 243 to 296 (54 residues), 27.6 bits, see alignment 2.5e-10 PF02518: HATPase_c" amino acids 347 to 447 (101 residues), 56.8 bits, see alignment E=2.9e-19

Best Hits

KEGG orthology group: K07637, two-component system, OmpR family, sensor histidine kinase PhoQ [EC: 2.7.13.3] (inferred from 100% identity to pfs:PFLU4719)

Predicted SEED Role

"Sensor protein PhoQ (EC 2.7.13.3)" in subsystem Lipid A modifications (EC 2.7.13.3)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K0P8 at UniProt or InterPro

Protein Sequence (448 amino acids)

>PFLU_RS23125 HAMP domain-containing protein (Pseudomonas fluorescens SBW25)
MIRSLRVRLMLAAATLAVLFMLGLLPAMQGAFSLALQDSIEQRLASDVTTLISAARVENN
RLLMPAQLPDERFNLTDSRLLGYIYDREGHLVWRSRATKEENINYKPRYDGRGNEFARIR
EANGQEFFVYDVEVRLLGGKSAAFSIVALQPVREYQLTLEGLRENLYLGFGAALLVLLTL
LWLGLTWGLQALRRLSQELDQIEGGTRESLSEQHPRELLRLTGSLNRLLHSEREQRTRYR
DSLDDLAHSLKTPLAVLQGVSEDMAQRPEDRDQAWVLQSQIERMSQQISYQLQRASLRKS
GLVRHQVRLAPVLQSLCDTLDKVYRDKRVTVSFELPEECDVPIEKGALLELLGNLLENAY
RLCLSEVGISLVESLEGTELCIEDDGPGVPPDQRARILQRGERLDRQHPGQGIGLAVVKD
IIESYGARLTLGDSPLGGAAFKIHFPAL