Protein Info for PFLU_RS23115 in Pseudomonas fluorescens SBW25

Annotation: dicarboxylate/amino acid:cation symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 443 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 45 to 66 (22 residues), see Phobius details amino acids 78 to 100 (23 residues), see Phobius details amino acids 147 to 169 (23 residues), see Phobius details amino acids 191 to 215 (25 residues), see Phobius details amino acids 221 to 242 (22 residues), see Phobius details amino acids 262 to 280 (19 residues), see Phobius details amino acids 300 to 319 (20 residues), see Phobius details amino acids 330 to 348 (19 residues), see Phobius details amino acids 353 to 376 (24 residues), see Phobius details amino acids 387 to 404 (18 residues), see Phobius details PF00375: SDF" amino acids 11 to 403 (393 residues), 392 bits, see alignment E=1.6e-121

Best Hits

Swiss-Prot: 100% identical to DCTA_PSEFS: C4-dicarboxylate transport protein (dctA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K11103, aerobic C4-dicarboxylate transport protein (inferred from 100% identity to pfs:PFLU4717)

MetaCyc: 78% identical to C4 dicarboxylate/orotate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-121; TRANS-RXN-121A; TRANS-RXN-121C; TRANS-RXN-122A; TRANS-RXN0-451; TRANS-RXN0-517; TRANS-RXN0-553

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K0P6 at UniProt or InterPro

Protein Sequence (443 amino acids)

>PFLU_RS23115 dicarboxylate/amino acid:cation symporter (Pseudomonas fluorescens SBW25)
MTTRQPIYKSLYFQVIVAIVIGILLGHFYPETGVALKPLGDGFIKLIKMVIAPIIFCTVV
SGIAGMQSMKSVGKTGGYALLYFEIVSTVALLIGLIVVNVVQPGAGMHIDVATLDASKVA
AYVTAGKDQSIVGFILNVIPNTIVGAFANGDILQVLMFSVIFGFALHRLGAYGKPVLDFI
DRFAHVMFNIINMIMKLAPIGALGAMAFTIGAYGVGSLVQLGQLMICFYITCLLFVLVVL
GGICRAHGFSVIKLIRYIREELLIVLGTSSSESALPRMLIKMERLGAKKSVVGLVIPTGY
SFNLDGTSIYLTMAAVFIAQATDTHMDITHQITLLLVLLLSSKGAAGVTGSGFIVLAATL
SAVGHLPVAGLALILGIDRFMSEARALTNLVGNAVATIVVAKWVKELDTDKLQSELASGG
TGISETREIDDLGVAEGPAPVVK